Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NLK52_RS17650 Genome accession   NZ_CP100436
Coordinates   3430727..3432118 (-) Length   463 a.a.
NCBI ID   WP_015384785.1    Uniprot ID   -
Organism   Bacillus subtilis strain MEC_B298     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3425727..3437118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLK52_RS17615 (NLK52_17615) flgL 3425898..3426794 (-) 897 WP_014478126.1 flagellar hook-associated protein FlgL -
  NLK52_RS17620 (NLK52_17620) flgK 3426805..3428328 (-) 1524 WP_015384782.1 flagellar hook-associated protein FlgK -
  NLK52_RS17625 (NLK52_17625) flgN 3428347..3428829 (-) 483 WP_014481048.1 flagellar protein FlgN -
  NLK52_RS17630 (NLK52_17630) flgM 3428845..3429111 (-) 267 WP_015384783.1 flagellar biosynthesis anti-sigma factor FlgM -
  NLK52_RS17635 (NLK52_17635) yvyF 3429192..3429611 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  NLK52_RS17640 (NLK52_17640) comFC 3429685..3430407 (-) 723 WP_015384784.1 comF operon protein ComFC Machinery gene
  NLK52_RS17645 (NLK52_17645) comFB 3430371..3430667 (-) 297 WP_015483764.1 late competence protein ComFB -
  NLK52_RS17650 (NLK52_17650) comFA 3430727..3432118 (-) 1392 WP_015384785.1 ATP-dependent helicase ComFA Machinery gene
  NLK52_RS17655 (NLK52_17655) fakBA 3432224..3433069 (-) 846 WP_003227986.1 DegV family protein -
  NLK52_RS17660 (NLK52_17660) degU 3433167..3433856 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  NLK52_RS17665 (NLK52_17665) degS 3433939..3435096 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  NLK52_RS17670 (NLK52_17670) yvyE 3435313..3435966 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52498.54 Da        Isoelectric Point: 9.9354

>NTDB_id=705459 NLK52_RS17650 WP_015384785.1 3430727..3432118(-) (comFA) [Bacillus subtilis strain MEC_B298]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLNLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=705459 NLK52_RS17650 WP_015384785.1 3430727..3432118(-) (comFA) [Bacillus subtilis strain MEC_B298]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGGAGCCGTCATTTGCTCAGGAC
TGAACTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGAAGTCAATTAAACTGACATGGGATGGCAAGCTTTCAAGCGGACAACAAAAAGCCGCCAATGTATTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATT
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGTTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCAATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.488

100

0.985