Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NLK52_RS12700 Genome accession   NZ_CP100436
Coordinates   2472064..2472474 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain MEC_B298     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2467064..2477474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLK52_RS12670 (NLK52_12670) - 2467151..2467291 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  NLK52_RS12675 (NLK52_12675) yqeF 2467597..2468328 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  NLK52_RS12680 (NLK52_12680) cwlH 2468580..2469332 (-) 753 WP_015483449.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  NLK52_RS12685 (NLK52_12685) yqeD 2469519..2470145 (+) 627 WP_015384137.1 TVP38/TMEM64 family protein -
  NLK52_RS12690 (NLK52_12690) gnd 2470164..2471057 (-) 894 WP_015384138.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NLK52_RS12695 (NLK52_12695) yqeB 2471309..2472031 (+) 723 WP_015384139.1 hypothetical protein -
  NLK52_RS12700 (NLK52_12700) nucA/comI 2472064..2472474 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  NLK52_RS12705 (NLK52_12705) sigK 2472670..2473398 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  NLK52_RS12710 (NLK52_12710) - 2473480..2473671 (-) 192 Protein_2461 recombinase family protein -
  NLK52_RS12715 (NLK52_12715) - 2473629..2473793 (-) 165 WP_015483452.1 hypothetical protein -
  NLK52_RS12720 (NLK52_12720) psiE 2474334..2474750 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  NLK52_RS12725 (NLK52_12725) yrkQ 2474796..2476094 (-) 1299 WP_015483453.1 two-component system sensor histidine kinase YrkQ -
  NLK52_RS12730 (NLK52_12730) yrkP 2476081..2476776 (-) 696 WP_015384143.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=705444 NLK52_RS12700 WP_009967785.1 2472064..2472474(-) (nucA/comI) [Bacillus subtilis strain MEC_B298]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=705444 NLK52_RS12700 WP_009967785.1 2472064..2472474(-) (nucA/comI) [Bacillus subtilis strain MEC_B298]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGTGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529