Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NLK52_RS12450 Genome accession   NZ_CP100436
Coordinates   2428623..2429390 (-) Length   255 a.a.
NCBI ID   WP_015384118.1    Uniprot ID   -
Organism   Bacillus subtilis strain MEC_B298     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2423623..2434390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLK52_RS12430 (NLK52_12430) ppsR 2423797..2424609 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  NLK52_RS12435 (NLK52_12435) ccpN 2424635..2425273 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  NLK52_RS12440 (NLK52_12440) glyS 2425406..2427445 (-) 2040 WP_015384117.1 glycine--tRNA ligase subunit beta -
  NLK52_RS12445 (NLK52_12445) glyQ 2427438..2428325 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  NLK52_RS12450 (NLK52_12450) recO 2428623..2429390 (-) 768 WP_015384118.1 DNA repair protein RecO Machinery gene
  NLK52_RS12455 (NLK52_12455) yqzL 2429427..2429570 (-) 144 WP_003230053.1 YqzL family protein -
  NLK52_RS12460 (NLK52_12460) era 2429718..2430623 (-) 906 WP_003230051.1 GTPase Era -
  NLK52_RS12465 (NLK52_12465) - 2430604..2431014 (-) 411 WP_003230049.1 cytidine deaminase -
  NLK52_RS12470 (NLK52_12470) unpK 2431133..2431504 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  NLK52_RS12475 (NLK52_12475) ybeY 2431485..2431958 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  NLK52_RS12480 (NLK52_12480) pgpH 2431959..2434094 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29332.01 Da        Isoelectric Point: 8.3080

>NTDB_id=705440 NLK52_RS12450 WP_015384118.1 2428623..2429390(-) (recO) [Bacillus subtilis strain MEC_B298]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKSNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=705440 NLK52_RS12450 WP_015384118.1 2428623..2429390(-) (recO) [Bacillus subtilis strain MEC_B298]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTATTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGTC
AAATCCTTATTTATTTGAATTCATACTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992