Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LZ581_RS20065 Genome accession   NZ_CP100388
Coordinates   4233399..4234832 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. steigerwaltii strain VKH10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4228399..4239832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ581_RS20035 (LZ581_20035) - 4228966..4229943 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  LZ581_RS20040 (LZ581_20040) kdsD 4229957..4230943 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  LZ581_RS20045 (LZ581_20045) kdsC 4230958..4231524 (+) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LZ581_RS20050 (LZ581_20050) lptC 4231521..4232096 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  LZ581_RS20055 (LZ581_20055) lptA 4232065..4232619 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LZ581_RS20060 (LZ581_20060) lptB 4232626..4233351 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  LZ581_RS20065 (LZ581_20065) rpoN/rpoN1 4233399..4234832 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  LZ581_RS20070 (LZ581_20070) hpf 4234855..4235142 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  LZ581_RS20075 (LZ581_20075) ptsN 4235225..4235716 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LZ581_RS20080 (LZ581_20080) rapZ 4235762..4236616 (+) 855 WP_003861870.1 RNase adapter RapZ -
  LZ581_RS20085 (LZ581_20085) npr 4236613..4236885 (+) 273 WP_040118154.1 PTS phosphocarrier protein NPr -
  LZ581_RS20090 (LZ581_20090) mtgA 4236925..4237650 (-) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LZ581_RS20095 (LZ581_20095) elbB 4237647..4238300 (-) 654 WP_032672055.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=704796 LZ581_RS20065 WP_003861864.1 4233399..4234832(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. steigerwaltii strain VKH10]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=704796 LZ581_RS20065 WP_003861864.1 4233399..4234832(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. steigerwaltii strain VKH10]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACTCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATCCCTGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATATGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48