Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   PDI73_RS00625 Genome accession   NZ_CP115479
Coordinates   132325..132966 (-) Length   213 a.a.
NCBI ID   WP_003131746.1    Uniprot ID   Q9CI22
Organism   Lactococcus lactis strain EP2     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 127325..137966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PDI73_RS00605 (PDI73_00605) cysK 127356..128288 (-) 933 WP_003131750.1 cysteine synthase A -
  PDI73_RS00610 (PDI73_00610) - 128506..129591 (+) 1086 WP_058206268.1 LysM peptidoglycan-binding domain-containing protein -
  PDI73_RS00615 (PDI73_00615) - 129630..130262 (-) 633 WP_033900522.1 MBL fold metallo-hydrolase -
  PDI73_RS00620 (PDI73_00620) - 130263..132257 (-) 1995 WP_033900537.1 transglycosylase domain-containing protein -
  PDI73_RS00625 (PDI73_00625) recU 132325..132966 (-) 642 WP_003131746.1 Holliday junction resolvase RecU Machinery gene
  PDI73_RS00630 (PDI73_00630) - 133047..133571 (+) 525 WP_010905434.1 DUF1273 domain-containing protein -
  PDI73_RS00635 (PDI73_00635) spx 133731..134117 (+) 387 WP_010905435.1 transcriptional regulator Spx -
  PDI73_RS00640 (PDI73_00640) - 134209..134490 (+) 282 WP_003131743.1 UPF0223 family protein -
  PDI73_RS00645 (PDI73_00645) - 134456..135235 (+) 780 WP_033900525.1 inositol monophosphatase family protein -
  PDI73_RS00650 (PDI73_00650) murA 135329..136615 (+) 1287 WP_033900539.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  PDI73_RS00655 (PDI73_00655) - 136615..136806 (+) 192 WP_033900527.1 DNA-directed RNA polymerase subunit beta -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 24369.13 Da        Isoelectric Point: 10.1344

>NTDB_id=704765 PDI73_RS00625 WP_003131746.1 132325..132966(-) (recU) [Lactococcus lactis strain EP2]
MVNYPNGRSRAYSTVPKKKKSLTELSVTKKSTSKTKGMVAFGKRGMNFEAEINATNEYYLSRGLAVIHKKPTPIQIVKVD
YPQRSRAKITEAYFRQASTTDYSGVYKGHYIDFEAKETQQKTVFPLKNFHEHQIVHMSNVLAQGGIAFVLLHFARLNETY
LLPSSCLITFYYEKGGLKSIPLSHIRENGYKIETNHIPRIPYLEIVNKLCEVQ

Nucleotide


Download         Length: 642 bp        

>NTDB_id=704765 PDI73_RS00625 WP_003131746.1 132325..132966(-) (recU) [Lactococcus lactis strain EP2]
ATGGTTAATTATCCTAATGGAAGGTCGCGAGCTTACAGCACTGTTCCTAAGAAAAAAAAATCACTGACAGAGCTGTCAGT
AACTAAAAAAAGCACCTCAAAAACCAAAGGGATGGTTGCCTTCGGGAAGCGGGGAATGAATTTTGAAGCTGAAATTAATG
CAACAAATGAGTATTATCTTTCTCGTGGACTTGCAGTAATTCACAAAAAGCCAACACCAATTCAAATCGTTAAAGTAGAT
TATCCACAAAGGAGTCGTGCAAAAATTACAGAAGCTTACTTCAGGCAAGCATCTACTACTGACTACTCTGGAGTCTATAA
AGGTCACTACATCGACTTTGAAGCAAAGGAAACACAGCAAAAAACTGTTTTCCCTCTAAAAAATTTCCATGAGCATCAAA
TTGTTCACATGAGTAATGTTCTCGCTCAAGGGGGAATTGCTTTTGTCCTCTTGCACTTTGCAAGGTTAAATGAAACATAT
CTTCTTCCATCTTCGTGTTTGATTACTTTTTATTACGAAAAAGGCGGTTTAAAGTCAATTCCGCTTTCTCATATTCGCGA
AAATGGCTATAAAATTGAAACGAACCACATTCCTAGAATTCCTTATCTAGAAATTGTGAATAAACTTTGTGAGGTACAGT
AA

Domains

Predicted by InterProScan.

Recombination protein U (RecU)

(44-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CI22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

47.393

99.061

0.469