Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   PCS88_RS24025 Genome accession   NZ_CP115390
Coordinates   4957252..4959081 (-) Length   609 a.a.
NCBI ID   WP_001442069.1    Uniprot ID   -
Organism   Escherichia coli strain CUVET20-PYO1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4952252..4964081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS88_RS24000 (PCS88_23965) yigL 4952949..4953749 (-) 801 WP_000285360.1 sugar/pyridoxal phosphate phosphatase YigL -
  PCS88_RS24005 (PCS88_23970) pldB 4953757..4954779 (-) 1023 WP_000487669.1 lysophospholipase L2 -
  PCS88_RS24010 (PCS88_23975) rhtB 4954890..4955510 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  PCS88_RS24015 (PCS88_23980) - 4955669..4956529 (+) 861 WP_000133649.1 Rpn family recombination-promoting nuclease/putative transposase -
  PCS88_RS24020 (PCS88_23985) rhtC 4956567..4957187 (-) 621 WP_000928838.1 threonine export protein RhtC -
  PCS88_RS24025 (PCS88_23990) recQ 4957252..4959081 (-) 1830 WP_001442069.1 ATP-dependent DNA helicase RecQ Machinery gene
  PCS88_RS24030 (PCS88_23995) pldA 4959206..4960075 (-) 870 WP_001259692.1 phospholipase A -
  PCS88_RS24035 (PCS88_24000) yigI 4960240..4960707 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  PCS88_RS24040 (PCS88_24005) rarD 4960759..4961649 (+) 891 WP_000339102.1 EamA family transporter RarD -
  PCS88_RS24045 (PCS88_24010) - 4961744..4962124 (+) 381 WP_001532065.1 hypothetical protein -
  PCS88_RS24050 (PCS88_24015) - 4962139..4962519 (+) 381 WP_000031247.1 DUF2628 domain-containing protein -
  PCS88_RS24055 (PCS88_24020) corA 4962562..4963512 (-) 951 WP_000947159.1 magnesium/cobalt transporter CorA -
  PCS88_RS24060 (PCS88_24025) ysgD 4963664..4963720 (-) 57 WP_212723238.1 YsgD/CorL family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68294.40 Da        Isoelectric Point: 7.0905

>NTDB_id=704227 PCS88_RS24025 WP_001442069.1 4957252..4959081(-) (recQ) [Escherichia coli strain CUVET20-PYO1]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=704227 PCS88_RS24025 WP_001442069.1 4957252..4959081(-) (recQ) [Escherichia coli strain CUVET20-PYO1]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACCGTACTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTCTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTAGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
GATGACAGGCTGTCGCACCGGGCAAATCAGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAGGCGCACTGTATCTCCCAATGGGGTCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGTTTTCCGACGCTGCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGTTATGTACAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGTAATAGTCGTGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCTGATGTGCAGGAAAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAACAAGCCTAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACTGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCGT
TTGCTGAAGCGCAAACTTGCCGTCGTTTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCAAAACAGTACGATGGTTCAACCGATGCGCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGCGGTGCTAATAATCAGCGCATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCTACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGTATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGTGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

HRDC domain (HRDC)

(533-599)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391