Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NIX86_RS15800 Genome accession   NZ_CP100262
Coordinates   3368890..3370401 (+) Length   503 a.a.
NCBI ID   WP_015877038.1    Uniprot ID   A0AAQ0BTD2
Organism   Burkholderia glumae strain BGR79S     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3363890..3375401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIX86_RS15775 kdsD 3365108..3366091 (+) 984 WP_015877033.1 arabinose 5-phosphate isomerase KdsD -
  NIX86_RS15780 - 3366091..3366630 (+) 540 WP_015877034.1 HAD family hydrolase -
  NIX86_RS15785 lptC 3366634..3367248 (+) 615 WP_161521123.1 LPS export ABC transporter periplasmic protein LptC -
  NIX86_RS15790 lptA 3367267..3367938 (+) 672 WP_015877036.1 lipopolysaccharide transport periplasmic protein LptA -
  NIX86_RS15795 lptB 3367935..3368711 (+) 777 WP_015877037.1 LPS export ABC transporter ATP-binding protein -
  NIX86_RS15800 rpoN/rpoN1 3368890..3370401 (+) 1512 WP_015877038.1 RNA polymerase factor sigma-54 Machinery gene
  NIX86_RS15805 raiA 3370553..3370912 (+) 360 WP_015877039.1 ribosome-associated translation inhibitor RaiA -
  NIX86_RS15810 ptsN 3371129..3371641 (+) 513 WP_015877040.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NIX86_RS15815 hprK 3371776..3372744 (+) 969 WP_015877041.1 HPr(Ser) kinase/phosphatase -
  NIX86_RS15820 rapZ 3372819..3373712 (+) 894 WP_015877042.1 RNase adapter RapZ -
  NIX86_RS15825 - 3373743..3374378 (+) 636 WP_257813164.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55057.11 Da        Isoelectric Point: 4.8365

>NTDB_id=704055 NIX86_RS15800 WP_015877038.1 3368890..3370401(+) (rpoN/rpoN1) [Burkholderia glumae strain BGR79S]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPSSAPEPMQ
GAPVGGDAPAGERSERDDGSGGDDYDSYSGSDGDASQWNLDDYGRTSGASDDDDLPPLQVHETSTSLRDHLSAQLRVTQA
SPRDRALITFLIESLDDDGYLGATLDEVRADLPEELEVELDELAAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTR
TLALDIVSQHLELLAARDFTRLRKQLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVKKSGQNWLAELNPEV
VPKLRINHLYANILRNSRGDPGAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADT
LGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=704055 NIX86_RS15800 WP_015877038.1 3368890..3370401(+) (rpoN/rpoN1) [Burkholderia glumae strain BGR79S]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCACCTCGCGCTGACTCCCCAGTTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAAAACGACGACGAGTGGA
TCGCGAGCCCGCTGCGCGTGGCCGCCGACGGCTCGCTGATCGCGCAGACGCCGCCGTCCTCGGCGCCCGAGCCGATGCAG
GGTGCGCCCGTCGGCGGCGACGCGCCGGCCGGCGAGCGCAGCGAGCGCGACGACGGCTCGGGCGGCGACGACTACGACAG
CTACTCCGGCAGCGACGGCGATGCGAGCCAGTGGAACCTCGACGACTACGGCCGCACTTCGGGCGCCTCCGACGACGACG
ATCTCCCGCCGCTGCAGGTCCACGAGACCTCGACTTCGCTGCGCGACCACCTGTCGGCACAGCTGCGCGTCACCCAGGCA
AGCCCGCGCGACCGCGCGCTGATCACGTTCCTGATCGAGTCGCTCGACGACGACGGCTATCTGGGCGCGACGCTCGACGA
AGTCCGCGCCGACCTGCCCGAGGAGCTCGAGGTCGAGCTCGACGAGCTGGCCGCCGCGCTCGCGCTGCTGCACAGCTTCG
ATCCGGCCGGCGTCGGCGCGCGTTCGGCGTCCGAATGCCTGCGCCTGCAGCTGCTGCGGCTCGATCCGTCCCCCACGCGC
ACGCTCGCGCTCGACATCGTTTCGCAGCATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAA
GGCGAGCGACGACGAGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAGCCGTTCCCGGGCGCCGCCTACGGCAAGG
CCGAGGCCGACTACGTGGTGCCCGACATCATGGTGAAGAAGAGCGGCCAGAACTGGCTCGCGGAGCTGAATCCGGAAGTC
GTGCCCAAGCTGCGCATCAACCACCTGTACGCCAACATCCTGCGCAACAGCCGCGGCGATCCCGGCGCCGGCTCGCTGAA
GCAGCAGCTCCAGGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGATTCGAGACCATCCTGCGCGTCGCGCAAGCAA
TCGTCGAGCGCCAGAAGAACTTTTTTGCACATGGCGAAATAGCCATGCGCCCCTTGGTTTTGAGGGAAATAGCTGATACG
CTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAA
GTACTTCTTCGGGTCGCATGTCTCGACGGATACCGGCGGCGCCGCCTCATCCACGGCGATCCGCGCCCTCATCAAGCAAC
TGATAGGAGCCGAAGACCCGAAAACTCCTTTATCGGACAGCCGAATCGCCGAGCTGCTGGCGGAACAGGGATTCGTGGTC
GCGCGCCGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-501)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.992

100

0.68