Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   PCS82_RS16430 Genome accession   NZ_CP115376
Coordinates   3400966..3402795 (+) Length   609 a.a.
NCBI ID   WP_024179510.1    Uniprot ID   -
Organism   Escherichia coli strain CUVET20-PYO4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3395966..3407795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS82_RS16395 (PCS82_16360) - 3396305..3397153 (-) 849 WP_022646337.1 YPO3983 family protein -
  PCS82_RS16400 (PCS82_16365) - 3397340..3397486 (+) 147 Protein_3214 CorA family divalent cation transporter -
  PCS82_RS16405 (PCS82_16370) yigF 3397529..3397909 (-) 381 Protein_3215 DUF2628 domain-containing protein -
  PCS82_RS16410 (PCS82_16375) yigG 3397923..3398303 (-) 381 WP_021521448.1 protein YigG -
  PCS82_RS16415 (PCS82_16380) rarD 3398398..3399288 (-) 891 WP_000339104.1 EamA family transporter RarD -
  PCS82_RS16420 (PCS82_16385) yigI 3399340..3399807 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  PCS82_RS16425 (PCS82_16390) pldA 3399972..3400841 (+) 870 WP_001259693.1 phospholipase A -
  PCS82_RS16430 (PCS82_16395) recQ 3400966..3402795 (+) 1830 WP_024179510.1 ATP-dependent DNA helicase RecQ Machinery gene
  PCS82_RS16435 (PCS82_16400) rhtC 3402859..3403479 (+) 621 WP_000928837.1 threonine export protein RhtC -
  PCS82_RS16440 (PCS82_16405) - 3403516..3404376 (-) 861 WP_000133649.1 Rpn family recombination-promoting nuclease/putative transposase -
  PCS82_RS16445 (PCS82_16410) rhtB 3404535..3405155 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  PCS82_RS16450 (PCS82_16415) pldB 3405266..3406288 (+) 1023 WP_022646339.1 lysophospholipase L2 -
  PCS82_RS16455 (PCS82_16420) yigL 3406296..3407096 (+) 801 WP_000285349.1 sugar/pyridoxal phosphate phosphatase YigL -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68391.56 Da        Isoelectric Point: 7.2543

>NTDB_id=704036 PCS82_RS16430 WP_024179510.1 3400966..3402795(+) (recQ) [Escherichia coli strain CUVET20-PYO4]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLDVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNIRVDVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=704036 PCS82_RS16430 WP_024179510.1 3400966..3402795(+) (recQ) [Escherichia coli strain CUVET20-PYO4]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGGGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACCGTACTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGACGT
GATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTGTATATCGCGCCGGAACGCCTGATGCTGGATAACTTCCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGTCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGGTTCCCGACGCTCCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGGCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGATCGCCCGAACATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGCAACAGCCGGGCGAAAGTCGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATATTCGCGTCGACGTGCAGGAAAAATTTCAGCGCGATGACCTGCAAATTGTGGTGGCGACCG
TGGCGTTTGGCATGGGCATCAACAAGCCTAACGTCCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTACGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAATTGCAGGATATCGAGCGCCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTGCTGCTGAACTACTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTATTTAACGAC
GCAACCTTGATTGAGATGGCGGAACAGATGCCGATCACTGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

39.865

97.209

0.388