Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NIV78_RS15220 Genome accession   NZ_CP100136
Coordinates   3252759..3254270 (+) Length   503 a.a.
NCBI ID   WP_015877038.1    Uniprot ID   A0AAQ0BTD2
Organism   Burkholderia glumae strain R93     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3247759..3259270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIV78_RS15195 kdsD 3248977..3249960 (+) 984 WP_015877033.1 arabinose 5-phosphate isomerase KdsD -
  NIV78_RS15200 - 3249960..3250499 (+) 540 WP_015877034.1 KdsC family phosphatase -
  NIV78_RS15205 lptC 3250503..3251117 (+) 615 WP_015877035.1 LPS export ABC transporter periplasmic protein LptC -
  NIV78_RS15210 lptA 3251136..3251807 (+) 672 WP_015877036.1 lipopolysaccharide transport periplasmic protein LptA -
  NIV78_RS15215 lptB 3251804..3252580 (+) 777 WP_015877037.1 LPS export ABC transporter ATP-binding protein -
  NIV78_RS15220 rpoN/rpoN1 3252759..3254270 (+) 1512 WP_015877038.1 RNA polymerase factor sigma-54 Machinery gene
  NIV78_RS15225 hpf 3254422..3254781 (+) 360 WP_015877039.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NIV78_RS15230 ptsN 3254998..3255510 (+) 513 WP_015877040.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NIV78_RS15235 hprK 3255645..3256613 (+) 969 WP_015877041.1 HPr(Ser) kinase/phosphatase -
  NIV78_RS15240 rapZ 3256688..3257581 (+) 894 WP_015877042.1 RNase adapter RapZ -
  NIV78_RS15245 - 3257612..3258247 (+) 636 WP_017432629.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55057.11 Da        Isoelectric Point: 4.8365

>NTDB_id=703750 NIV78_RS15220 WP_015877038.1 3252759..3254270(+) (rpoN/rpoN1) [Burkholderia glumae strain R93]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPSSAPEPMQ
GAPVGGDAPAGERSERDDGSGGDDYDSYSGSDGDASQWNLDDYGRTSGASDDDDLPPLQVHETSTSLRDHLSAQLRVTQA
SPRDRALITFLIESLDDDGYLGATLDEVRADLPEELEVELDELAAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTR
TLALDIVSQHLELLAARDFTRLRKQLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVKKSGQNWLAELNPEV
VPKLRINHLYANILRNSRGDPGAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADT
LGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=703750 NIV78_RS15220 WP_015877038.1 3252759..3254270(+) (rpoN/rpoN1) [Burkholderia glumae strain R93]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCACCTCGCGCTGACTCCCCAGTTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAAAACGACGACGAGTGGA
TCGCGAGCCCGCTGCGCGTGGCCGCCGACGGCTCGCTGATCGCGCAGACGCCGCCGTCCTCGGCGCCCGAGCCGATGCAG
GGTGCGCCCGTCGGCGGCGACGCGCCGGCCGGCGAGCGCAGCGAGCGCGACGACGGCTCGGGCGGCGACGACTACGACAG
CTACTCCGGCAGCGACGGCGATGCGAGCCAGTGGAACCTCGACGACTACGGCCGCACTTCGGGCGCCTCCGACGACGACG
ATCTCCCGCCGCTGCAGGTCCACGAGACCTCGACTTCGCTGCGCGACCACCTGTCGGCACAGCTGCGCGTCACCCAGGCA
AGCCCGCGCGACCGCGCGCTGATCACGTTCCTGATCGAGTCGCTCGACGACGACGGCTATCTGGGCGCGACGCTCGACGA
AGTCCGCGCCGACCTGCCCGAGGAGCTCGAGGTCGAGCTCGACGAGCTGGCCGCCGCGCTCGCGCTGCTGCACAGCTTCG
ATCCGGCCGGCGTCGGCGCGCGTTCGGCGTCCGAATGCCTGCGCCTGCAGCTGCTGCGACTCGATCCGTCCCCCACGCGC
ACGCTCGCGCTCGACATCGTTTCGCAGCATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAA
GGCGAGCGACGACGAGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAGCCGTTCCCGGGCGCCGCCTACGGCAAGG
CCGAGGCCGACTACGTGGTGCCCGACATCATGGTGAAGAAGAGCGGCCAGAACTGGCTCGCGGAGCTGAATCCGGAAGTC
GTGCCCAAGCTGCGCATCAACCACCTGTACGCCAACATCCTGCGCAACAGCCGCGGCGATCCCGGCGCCGGCTCGCTGAA
GCAGCAGCTCCAGGAAGCGCGCTGGCTGATCAAAAACATCCAGCAGCGATTCGAGACCATCCTGCGCGTCGCGCAAGCAA
TCGTCGAGCGCCAGAAGAACTTTTTTGCACATGGCGAAATAGCCATGCGCCCCTTGGTTTTGAGGGAAATAGCTGATACG
CTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAA
GTACTTCTTCGGGTCGCATGTCTCGACGGATACCGGCGGCGCCGCCTCATCCACGGCGATCCGCGCCCTCATCAAGCAAC
TGATAGGAGCCGAAGACCCGAAAACTCCTTTATCGGACAGCCGAATCGCCGAGCTGCTGGCGGAACAGGGATTCGTGGTC
GCGCGCCGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-501)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.992

100

0.68