Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NKT35_RS16425 Genome accession   NZ_CP100128
Coordinates   3522584..3523945 (+) Length   453 a.a.
NCBI ID   WP_254294944.1    Uniprot ID   -
Organism   Chromobacterium sp. IIBBL 290-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3517584..3528945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKT35_RS16400 (NKT35_16400) hpnC 3518187..3518999 (+) 813 WP_254294935.1 squalene synthase HpnC -
  NKT35_RS16405 (NKT35_16405) hpnD 3519062..3519907 (+) 846 WP_254294936.1 presqualene diphosphate synthase HpnD -
  NKT35_RS16410 (NKT35_16410) hpnE 3519874..3521178 (+) 1305 WP_254294938.1 hydroxysqualene dehydroxylase HpnE -
  NKT35_RS16415 (NKT35_16415) - 3521175..3521933 (+) 759 WP_254294940.1 SDR family oxidoreductase -
  NKT35_RS16420 (NKT35_16420) - 3521950..3522513 (+) 564 WP_254294942.1 hypothetical protein -
  NKT35_RS16425 (NKT35_16425) radA 3522584..3523945 (+) 1362 WP_254294944.1 DNA repair protein RadA Machinery gene
  NKT35_RS16430 (NKT35_16430) - 3523949..3524509 (+) 561 WP_254294946.1 sigma-70 family RNA polymerase sigma factor -
  NKT35_RS16435 (NKT35_16435) - 3524506..3524688 (+) 183 WP_256493408.1 zf-HC2 domain-containing protein -
  NKT35_RS16440 (NKT35_16440) - 3524775..3525668 (+) 894 WP_254294948.1 hypothetical protein -
  NKT35_RS16445 (NKT35_16445) - 3525807..3526736 (-) 930 WP_254294950.1 CysB family HTH-type transcriptional regulator -
  NKT35_RS16450 (NKT35_16450) - 3526733..3527806 (-) 1074 WP_254294951.1 sulfate/molybdate ABC transporter ATP-binding protein -
  NKT35_RS16455 (NKT35_16455) cysW 3527808..3528686 (-) 879 WP_254294953.1 sulfate ABC transporter permease subunit CysW -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48671.96 Da        Isoelectric Point: 7.0437

>NTDB_id=703719 NKT35_RS16425 WP_254294944.1 3522584..3523945(+) (radA) [Chromobacterium sp. IIBBL 290-4]
MAKSKTVFSCTECGGQSPKWQGQCPHCNAWNTLTEAVATPAAASPRFQSWAANVTKVQKLSEVQTAETPRDPSGIEELDR
VLGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGQNRKVLYVSGEESAQQIALRASRLALDTSSVDLLAEICIENILATL
KREQPEVVVIDSIQTLYTEQVTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
SHSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEGSRPLLVEIQALVDDCHGFQP
KRLTVGLEQNRLAMLLAVLHRHGGVACFDQDVFLNAVGGVKINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEV
RPVTRGQERLKEAAKLGFTRAIVPSANKPRQEIEGLKVLAVDRLDQAVEFCRE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=703719 NKT35_RS16425 WP_254294944.1 3522584..3523945(+) (radA) [Chromobacterium sp. IIBBL 290-4]
ATGGCCAAGAGCAAAACCGTATTCAGCTGCACCGAGTGCGGCGGACAATCGCCCAAGTGGCAGGGCCAGTGCCCGCATTG
CAACGCCTGGAACACGCTGACGGAGGCGGTGGCGACGCCGGCCGCCGCCAGCCCGCGCTTCCAGTCCTGGGCGGCCAACG
TCACCAAGGTCCAGAAACTGTCCGAGGTGCAAACCGCGGAAACCCCGCGCGATCCTTCCGGCATTGAAGAACTGGACAGG
GTGCTGGGCGGCGGCATCGTCCGCGGCGCCGTGGTGCTCATCGGCGGCGACCCCGGCATCGGCAAATCCACCTTGCTGCT
GCAAGCCTTGTCGCAGATCGGCCAGAACCGCAAGGTGCTGTATGTGTCGGGCGAGGAATCGGCGCAGCAAATCGCCTTGC
GCGCTTCGCGCCTGGCGCTGGATACCAGCAGCGTCGATCTGCTGGCCGAAATCTGCATCGAAAACATCCTGGCCACGCTA
AAGCGCGAACAGCCCGAGGTGGTGGTGATCGACTCCATCCAGACGCTGTACACCGAGCAGGTGACTTCGGCGCCTGGTTC
CGTGTCGCAGGTGCGCGAGTGCGCGGCGCAGCTGACGCGTATGGCCAAGCAAAGCGGCATCACTGTGCTGCTGGTCGGCC
ACGTCACCAAGGAAGGCTCGCTGGCCGGTCCGCGGGTGCTGGAGCATATGGTGGACACGGTGCTGTATTTCGAGGGCGAT
TCGCACTCCAGCTACCGCATGATACGCGCGATCAAGAACCGCTTCGGCGCGGTCAATGAGCTGGGCGTGTTCGCGATGAC
CGACCGCGGCCTCAAAGGCGTATCCAATCCTTCCGCCATTTTTCTGTCGTCCTATCGCGACGATGTCGCCGGTTCTTGCG
TGCTGGTGACGCAGGAAGGCTCGCGCCCGCTGTTGGTGGAAATCCAGGCCCTGGTGGATGACTGCCATGGCTTCCAGCCC
AAGCGGCTGACGGTTGGCCTGGAGCAGAACCGGCTGGCCATGCTGCTGGCGGTGCTGCATCGCCATGGCGGCGTGGCTTG
CTTCGATCAGGACGTGTTCCTTAACGCGGTGGGCGGCGTCAAGATCAACGAGCCGGCGGCCGACCTGGCCATCATCCTGG
CCATGGTTTCATCGCTGCGCAACAAGGCGCTGCCGGAGAAGCTGGTGGTGTTCGGCGAAGTGGGCCTGGCAGGAGAGGTG
CGGCCGGTGACGCGCGGCCAGGAGCGCTTGAAAGAGGCAGCCAAGCTGGGCTTCACCCGGGCCATCGTGCCATCCGCCAA
CAAGCCGCGCCAAGAGATAGAAGGCCTGAAGGTATTGGCGGTGGACCGTCTGGACCAAGCGGTGGAATTCTGCCGGGAGT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-219)

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.781

100

0.501
  radA Streptococcus mitis SK321

48.009

99.779

0.479
  radA Streptococcus mitis NCTC 12261

47.788

99.779

0.477
  radA Streptococcus pneumoniae TIGR4

50

93.598

0.468
  radA Streptococcus pneumoniae D39

50

93.598

0.468
  radA Streptococcus pneumoniae Rx1

50

93.598

0.468
  radA Streptococcus pneumoniae R6

50

93.598

0.468