Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NKT35_RS00230 Genome accession   NZ_CP100128
Coordinates   53611..55005 (+) Length   464 a.a.
NCBI ID   WP_254297803.1    Uniprot ID   -
Organism   Chromobacterium sp. IIBBL 290-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 48611..60005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKT35_RS00205 (NKT35_00205) - 50213..51193 (+) 981 WP_254297798.1 SIS domain-containing protein -
  NKT35_RS00210 (NKT35_00210) - 51236..51760 (+) 525 WP_254297799.1 HAD family hydrolase -
  NKT35_RS00215 (NKT35_00215) lptC 51757..52326 (+) 570 WP_254297800.1 LPS export ABC transporter periplasmic protein LptC -
  NKT35_RS00220 (NKT35_00220) lptA 52316..52876 (+) 561 WP_254297801.1 lipopolysaccharide transport periplasmic protein LptA -
  NKT35_RS00225 (NKT35_00225) lptB 52879..53607 (+) 729 WP_371926518.1 LPS export ABC transporter ATP-binding protein -
  NKT35_RS00230 (NKT35_00230) rpoN/rpoN1 53611..55005 (+) 1395 WP_254297803.1 RNA polymerase factor sigma-54 Machinery gene
  NKT35_RS00235 (NKT35_00235) hpf 55075..55407 (+) 333 WP_254297804.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NKT35_RS00240 (NKT35_00240) ptsN 55532..55993 (+) 462 WP_254297805.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NKT35_RS00245 (NKT35_00245) hprK 55986..56942 (+) 957 WP_254297806.1 HPr(Ser) kinase/phosphatase -
  NKT35_RS00250 (NKT35_00250) rapZ 56923..57765 (+) 843 WP_254297807.1 RNase adapter RapZ -
  NKT35_RS00255 (NKT35_00255) - 57817..58428 (+) 612 WP_254297808.1 flavin prenyltransferase UbiX -
  NKT35_RS00260 (NKT35_00260) - 58431..59897 (+) 1467 WP_254297809.1 NAD(P)H-hydrate dehydratase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 51550.56 Da        Isoelectric Point: 4.7393

>NTDB_id=703699 NKT35_RS00230 WP_254297803.1 53611..55005(+) (rpoN/rpoN1) [Chromobacterium sp. IIBBL 290-4]
MKQALQLKVSQQLTLTPQLQQSIKLLQLSTLDLQAEVERFLLDNPLLEHSDEPREADSPSDREDAPAESAADDARDGDGG
ELLEWGGGSRAAGDDDFDPMLNVPCQVTLREHLLAQLGEVALSDRDRAIMRLLIEELDDDGYLPVQLEELASNIPLELEL
DSDELAVGLRLLQQFDPAGVGARSLAEALELQLLRRPGQEPGHALALTIVREHLALLGNRDYARLKKTLGCGDDQLREAQ
TQIASLNPRPAAGFVEGEVHYVIPDISVRKRRGRWVAELNRAAMPKLRVNGLYERMLADNRGQNGEMSGRLQEARWLVKN
IQQRFDTILKVAEAIVERQQLFFEHGEVAMRPLILRDIAEEIGLHESTVSRSTSQKYLLCARGLFELKYFFGSSLETESG
GECSATAIKAHIRGMVESENPAKPLSDSAIADALDKLGIQVARRTVAKYREAMQIPPVNLRKTL

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=703699 NKT35_RS00230 WP_254297803.1 53611..55005(+) (rpoN/rpoN1) [Chromobacterium sp. IIBBL 290-4]
ATGAAACAGGCCCTCCAGCTCAAAGTTTCCCAGCAGCTGACGCTGACGCCCCAACTGCAACAATCGATCAAATTGCTGCA
GCTCTCCACGCTGGATCTGCAAGCGGAAGTCGAGCGTTTTCTGCTCGACAACCCCTTGCTGGAGCACAGCGACGAGCCGC
GCGAAGCCGATAGCCCCTCCGACCGGGAAGACGCGCCGGCGGAAAGCGCGGCGGACGATGCGCGAGACGGCGATGGCGGC
GAATTGCTGGAATGGGGCGGCGGCAGCCGCGCCGCCGGCGACGACGACTTCGACCCCATGCTGAACGTGCCGTGCCAGGT
CACCCTGCGCGAGCATCTGCTGGCGCAGTTGGGCGAGGTGGCGCTCTCGGATCGCGATCGGGCCATCATGCGCCTGCTGA
TCGAAGAGTTGGACGATGACGGCTATCTGCCGGTGCAGCTGGAAGAGCTGGCCAGCAATATCCCGCTGGAGCTGGAGCTG
GACAGCGACGAGCTGGCGGTGGGACTGCGCCTGCTGCAGCAGTTCGACCCCGCCGGCGTGGGCGCGCGCTCGCTGGCCGA
GGCGCTGGAGCTGCAGCTGCTGCGCCGTCCCGGCCAAGAACCGGGACACGCCCTGGCGCTGACTATCGTCCGCGAGCATC
TCGCGCTGCTGGGCAACCGCGACTACGCCCGCTTGAAAAAAACGCTGGGCTGCGGCGACGATCAGCTGCGCGAAGCGCAA
ACGCAGATCGCCAGCCTCAATCCCCGCCCGGCCGCCGGCTTCGTCGAGGGCGAGGTCCATTACGTGATTCCCGACATCAG
CGTGCGCAAACGCCGCGGCCGCTGGGTGGCCGAACTCAACCGCGCCGCCATGCCCAAGCTGCGCGTCAACGGGCTGTATG
AGCGCATGCTGGCCGACAACCGCGGCCAGAACGGCGAAATGTCGGGGCGGCTTCAGGAGGCGCGCTGGCTGGTGAAAAAC
ATTCAACAGCGGTTTGACACCATCCTGAAAGTGGCGGAAGCTATTGTTGAACGTCAACAATTGTTCTTTGAACATGGCGA
AGTGGCGATGCGCCCGCTGATCCTGCGCGACATCGCCGAAGAAATCGGCCTGCACGAATCCACCGTCTCGCGCTCGACCA
GCCAGAAGTATCTGCTATGCGCGCGAGGTTTGTTCGAGCTCAAGTATTTCTTCGGCAGTTCGCTGGAAACTGAAAGCGGC
GGAGAGTGCTCCGCCACGGCGATCAAGGCCCACATCCGCGGCATGGTGGAGTCGGAAAATCCGGCCAAGCCGCTGTCGGA
CAGCGCCATCGCCGATGCGCTGGACAAGCTGGGAATACAGGTTGCCAGACGCACCGTAGCCAAGTATCGTGAAGCCATGC
AGATTCCTCCCGTGAATCTGCGCAAGACACTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-293)

Sigma-54, DNA binding domain (Sigma54_DBD)

(309-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.199

100

0.543