Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PCS86_RS21180 Genome accession   NZ_CP115343
Coordinates   4323940..4325373 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain CUVET21-H2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4318940..4330373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS86_RS21150 (PCS86_21085) yrbG 4319498..4320475 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  PCS86_RS21155 (PCS86_21090) kdsD 4320489..4321475 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PCS86_RS21160 (PCS86_21095) kdsC 4321496..4322062 (+) 567 WP_000030015.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PCS86_RS21165 (PCS86_21100) lptC 4322059..4322634 (+) 576 WP_001539833.1 LPS export ABC transporter periplasmic protein LptC -
  PCS86_RS21170 (PCS86_21105) lptA 4322603..4323160 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PCS86_RS21175 (PCS86_21110) lptB 4323167..4323892 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PCS86_RS21180 (PCS86_21115) rpoN/rpoN1 4323940..4325373 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  PCS86_RS21185 (PCS86_21120) hpf 4325396..4325683 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PCS86_RS21190 (PCS86_21125) ptsN 4325801..4326292 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PCS86_RS21195 (PCS86_21130) rapZ 4326338..4327192 (+) 855 WP_000243741.1 RNase adapter RapZ -
  PCS86_RS21200 (PCS86_21135) npr 4327189..4327461 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PCS86_RS21205 (PCS86_21140) yrbL 4327675..4328307 (+) 633 WP_001539838.1 PhoP regulatory network protein YrbL -
  PCS86_RS21210 (PCS86_21145) mtgA 4328304..4329032 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PCS86_RS21215 (PCS86_21150) elbB 4329029..4329682 (-) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=703555 PCS86_RS21180 WP_000809051.1 4323940..4325373(+) (rpoN/rpoN1) [Escherichia coli strain CUVET21-H2]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=703555 PCS86_RS21180 WP_000809051.1 4323940..4325373(+) (rpoN/rpoN1) [Escherichia coli strain CUVET21-H2]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGATACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAAAGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465