Detailed information NTG0703545

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: PCS86_RS18405
• Genome accession: NZ_CP115343
• Coordinates: 3739786..3740778 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain CUVET21-H2
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 3734786..3745778

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCS86_RS18385 (PCS86_18330)	ygbM	3735417..3736193 (+)	777	WP_001539449.1	HPr family phosphocarrier protein	-
PCS86_RS18390 (PCS86_18335)	ygbN	3736282..3737646 (+)	1365	WP_000104433.1	GntP family transporter	-
PCS86_RS18395 (PCS86_18340)	-	3737789..3738832 (-)	1044	WP_001161578.1	MBL fold metallo-hydrolase	-
PCS86_RS18400 (PCS86_18345)	-	3739057..3739608 (+)	552	WP_001179442.1	flavodoxin family protein	-
PCS86_RS18405 (PCS86_18350)	rpoS	3739786..3740778 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
PCS86_RS18410 (PCS86_18355)	nlpD	3740841..3741935 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
PCS86_RS18415 (PCS86_18360)	pcm	3742120..3742746 (-)	627	WP_000254701.1	protein-L-isoaspartate O-methyltransferase	-
PCS86_RS18420 (PCS86_18365)	surE	3742740..3743501 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
PCS86_RS18425 (PCS86_18370)	truD	3743482..3744531 (-)	1050	WP_000568931.1	tRNA pseudouridine(13) synthase TruD	-
PCS86_RS18430 (PCS86_18375)	ispF	3744528..3745007 (-)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
PCS86_RS18435 (PCS86_18380)	ispD	3745007..3745717 (-)	711	WP_001539454.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=703545 PCS86_RS18405 WP_000081550.1 3739786..3740778(-) (rpoS) [Escherichia coli strain CUVET21-H2]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=703545 PCS86_RS18405 WP_000081550.1 3739786..3740778(-) (rpoS) [Escherichia coli strain CUVET21-H2]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715