Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PCS96_RS18355 Genome accession   NZ_CP115330
Coordinates   3823112..3824545 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain CUVET16-394     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3818112..3829545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS96_RS18320 (PCS96_18275) elbB 3818804..3819457 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  PCS96_RS18325 (PCS96_18280) mtgA 3819454..3820182 (+) 729 WP_000047064.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PCS96_RS18330 (PCS96_18285) yrbL 3820179..3820811 (-) 633 WP_000620396.1 PhoP regulatory network protein YrbL -
  PCS96_RS18335 (PCS96_18290) npr 3821024..3821296 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PCS96_RS18340 (PCS96_18295) rapZ 3821293..3822147 (-) 855 WP_000243741.1 RNase adapter RapZ -
  PCS96_RS18345 (PCS96_18300) ptsN 3822193..3822684 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PCS96_RS18350 (PCS96_18305) hpf 3822802..3823089 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PCS96_RS18355 (PCS96_18310) rpoN/rpoN1 3823112..3824545 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  PCS96_RS18360 (PCS96_18315) lptB 3824593..3825318 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PCS96_RS18365 (PCS96_18320) lptA 3825325..3825882 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PCS96_RS18370 (PCS96_18325) lptC 3825851..3826426 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  PCS96_RS18375 (PCS96_18330) kdsC 3826423..3826989 (-) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PCS96_RS18380 (PCS96_18335) kdsD 3827010..3827996 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PCS96_RS18385 (PCS96_18340) yrbG 3828010..3828987 (-) 978 WP_001551707.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=703387 PCS96_RS18355 WP_000809051.1 3823112..3824545(-) (rpoN/rpoN1) [Escherichia coli strain CUVET16-394]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=703387 PCS96_RS18355 WP_000809051.1 3823112..3824545(-) (rpoN/rpoN1) [Escherichia coli strain CUVET16-394]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CTGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCACTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCAGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTTAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465