Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NHG71_RS13845 Genome accession   NZ_CP100040
Coordinates   2840755..2841192 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain B1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2835755..2846192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHG71_RS13830 (NHG71_13815) - 2838615..2838977 (-) 363 WP_007409352.1 helix-turn-helix domain-containing protein -
  NHG71_RS13835 (NHG71_13820) hxlA 2839209..2839844 (+) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  NHG71_RS13840 (NHG71_13825) hxlB 2839841..2840398 (+) 558 WP_032872811.1 6-phospho-3-hexuloisomerase -
  NHG71_RS13845 (NHG71_13830) nucA/comI 2840755..2841192 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NHG71_RS13850 (NHG71_13835) nin/comJ 2841213..2841611 (+) 399 WP_032872809.1 competence protein ComJ Regulator
  NHG71_RS13855 (NHG71_13840) - 2841652..2841870 (-) 219 Protein_2710 family 1 glycosylhydrolase -
  NHG71_RS19550 - 2841795..2842148 (-) 354 WP_079891133.1 family 1 glycosylhydrolase -
  NHG71_RS13860 (NHG71_13845) - 2842090..2842413 (-) 324 WP_032872805.1 YckD family protein -
  NHG71_RS13865 (NHG71_13850) - 2842486..2842920 (-) 435 WP_021494203.1 RDD family protein -
  NHG71_RS13870 (NHG71_13855) - 2842935..2844071 (-) 1137 WP_032872802.1 zinc-dependent alcohol dehydrogenase -
  NHG71_RS13875 (NHG71_13860) - 2844136..2845338 (-) 1203 WP_032872800.1 GTP-binding protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=703350 NHG71_RS13845 WP_003156588.1 2840755..2841192(+) (nucA/comI) [Bacillus velezensis strain B1]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=703350 NHG71_RS13845 WP_003156588.1 2840755..2841192(+) (nucA/comI) [Bacillus velezensis strain B1]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAAACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641