Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   PCS80_RS14250 Genome accession   NZ_CP115324
Coordinates   2892692..2894521 (+) Length   609 a.a.
NCBI ID   WP_001442069.1    Uniprot ID   -
Organism   Escherichia coli strain CUVET17-1136     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2887692..2899521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS80_RS14215 (PCS80_14200) ysgD 2888053..2888109 (+) 57 WP_211180520.1 protein YsgD -
  PCS80_RS14220 (PCS80_14205) corA 2888261..2889211 (+) 951 WP_000947159.1 magnesium/cobalt transporter CorA -
  PCS80_RS14225 (PCS80_14210) - 2889254..2889634 (-) 381 WP_000031247.1 DUF2628 domain-containing protein -
  PCS80_RS14230 (PCS80_14215) - 2889649..2890029 (-) 381 WP_001532065.1 hypothetical protein -
  PCS80_RS14235 (PCS80_14220) rarD 2890124..2891014 (-) 891 WP_000339102.1 EamA family transporter RarD -
  PCS80_RS14240 (PCS80_14225) yigI 2891066..2891533 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  PCS80_RS14245 (PCS80_14230) pldA 2891698..2892567 (+) 870 WP_001259692.1 phospholipase A -
  PCS80_RS14250 (PCS80_14235) recQ 2892692..2894521 (+) 1830 WP_001442069.1 ATP-dependent DNA helicase RecQ Machinery gene
  PCS80_RS14255 (PCS80_14240) rhtC 2894586..2895206 (+) 621 WP_000928838.1 threonine export protein RhtC -
  PCS80_RS14260 (PCS80_14245) - 2895244..2896104 (-) 861 WP_000133649.1 Rpn family recombination-promoting nuclease/putative transposase -
  PCS80_RS14265 (PCS80_14250) rhtB 2896263..2896883 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  PCS80_RS14270 (PCS80_14255) pldB 2896994..2898016 (+) 1023 WP_000487669.1 lysophospholipase L2 -
  PCS80_RS14275 (PCS80_14260) yigL 2898024..2898824 (+) 801 WP_000285360.1 sugar/pyridoxal phosphate phosphatase YigL -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68294.40 Da        Isoelectric Point: 7.0905

>NTDB_id=703292 PCS80_RS14250 WP_001442069.1 2892692..2894521(+) (recQ) [Escherichia coli strain CUVET17-1136]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=703292 PCS80_RS14250 WP_001442069.1 2892692..2894521(+) (recQ) [Escherichia coli strain CUVET17-1136]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACCGTACTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTCTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTAGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
GATGACAGGCTGTCGCACCGGGCAAATCAGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAGGCGCACTGTATCTCCCAATGGGGTCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGTTTTCCGACGCTGCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGTTATGTACAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGTAATAGTCGTGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCTGATGTGCAGGAAAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAACAAGCCTAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACTGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCGT
TTGCTGAAGCGCAAACTTGCCGTCGTTTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCAAAACAGTACGATGGTTCAACCGATGCGCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGCGGTGCTAATAATCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCTACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGTATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGTGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

HRDC domain (HRDC)

(533-599)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391