Detailed information NTG0703263

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: PCS80_RS08060
• Genome accession: NZ_CP115324
• Coordinates: 1612580..1613572 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain CUVET17-1136
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1607580..1618572

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCS80_RS08040 (PCS80_08030)	ygbM	1608218..1608994 (+)	777	WP_001136914.1	HPr family phosphocarrier protein	-
PCS80_RS08045 (PCS80_08035)	ygbN	1609083..1610447 (+)	1365	WP_000104433.1	GntP family transporter	-
PCS80_RS08050 (PCS80_08040)	-	1610590..1611633 (-)	1044	WP_001161583.1	MBL fold metallo-hydrolase	-
PCS80_RS08055 (PCS80_08045)	-	1611858..1612409 (+)	552	WP_001179444.1	flavodoxin family protein	-
PCS80_RS08060 (PCS80_08050)	rpoS	1612580..1613572 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
PCS80_RS08065 (PCS80_08055)	nlpD	1613635..1614729 (-)	1095	WP_306278776.1	murein hydrolase activator NlpD	-
PCS80_RS08070 (PCS80_08060)	pcm	1614914..1615540 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
PCS80_RS08075 (PCS80_08065)	surE	1615534..1616295 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
PCS80_RS08080 (PCS80_08070)	truD	1616276..1617325 (-)	1050	WP_000568920.1	tRNA pseudouridine(13) synthase TruD	-
PCS80_RS08085 (PCS80_08075)	ispF	1617322..1617801 (-)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
PCS80_RS08090 (PCS80_08080)	ispD	1617801..1618511 (-)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=703263 PCS80_RS08060 WP_000081550.1 1612580..1613572(-) (rpoS) [Escherichia coli strain CUVET17-1136]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=703263 PCS80_RS08060 WP_000081550.1 1612580..1613572(-) (rpoS) [Escherichia coli strain CUVET17-1136]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCATTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715