Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   PCS83_RS23860 Genome accession   NZ_CP115320
Coordinates   4878490..4880487 (+) Length   665 a.a.
NCBI ID   WP_001774090.1    Uniprot ID   -
Organism   Escherichia coli strain CUVET18-565     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4873490..4885487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS83_RS23835 (PCS83_23775) - 4874140..4874385 (-) 246 WP_001298603.1 hypothetical protein -
  PCS83_RS23840 (PCS83_23780) - 4874460..4874717 (-) 258 WP_000156431.1 hypothetical protein -
  PCS83_RS23845 (PCS83_23785) tssD 4874714..4875847 (-) 1134 WP_001540226.1 type VI secretion system tube protein TssD -
  PCS83_RS23850 (PCS83_23790) hxlB 4876332..4876889 (+) 558 WP_000339042.1 6-phospho-3-hexuloisomerase -
  PCS83_RS23855 (PCS83_23795) ptsG 4876931..4878439 (+) 1509 WP_000037970.1 glucose-specific PTS transporter subunit IIBC -
  PCS83_RS23860 (PCS83_23800) recP/tkt 4878490..4880487 (+) 1998 WP_001774090.1 transketolase Machinery gene
  PCS83_RS23865 (PCS83_23805) - 4880517..4881362 (-) 846 WP_001060029.1 MurR/RpiR family transcriptional regulator -
  PCS83_RS23870 (PCS83_23810) - 4881563..4882468 (-) 906 WP_000470719.1 LysR substrate-binding domain-containing protein -
  PCS83_RS23875 (PCS83_23815) - 4882546..4883769 (-) 1224 WP_001227362.1 cytosine permease -
  PCS83_RS23880 (PCS83_23820) - 4883795..4884184 (-) 390 WP_000214035.1 glutamyl-tRNA amidotransferase -
  PCS83_RS23885 (PCS83_23825) arcC 4884201..4885157 (-) 957 WP_001540234.1 carbamate kinase -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 73006.63 Da        Isoelectric Point: 5.7486

>NTDB_id=703239 PCS83_RS23860 WP_001774090.1 4878490..4880487(+) (recP/tkt) [Escherichia coli strain CUVET18-565]
MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHLRHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYD
LSMDDIRDFRQLHSRTPGHPEYGYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGRFAEDTAARFRAYGWHVIEGVDGHDPEEVDAAVREAKSVTDKPSLL
CCKTIIGFGSPNKANSHDCHGSALGADEVALVRERLQWSYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPEL
AAEFTRRMKGDLPAGWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKSVDFTGENP
AGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAALMKIRSVFVYTHDTIGLGEDGPTHQPVEQLA
SLRLTPNMETWRGCDQVEVAVAWQQAIERKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSE
IELVVSAAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVGLDGKVIGMTSFGESAP
ANVLFKHFGFTPENVLAQARELLNS

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=703239 PCS83_RS23860 WP_001774090.1 4878490..4880487(+) (recP/tkt) [Escherichia coli strain CUVET18-565]
ATGAATTCACAATTACTGGCTAATGCCATCCGAATGTTAAGTGTTGATGCGATCCAGAAAGCGAACTCCGGTCATCCGGG
CGCGCCGATGGGGATGGCGGATATTGCCGAAGTGGTCTGGCGTCGTCACCTGCGTCATAACCCAAAAAATCCGCAGTGGT
TTAACCGCGACCGCTATGTTCAGTCGAACGGTCATGGCTCCATGTTGATTTACGCTTTGCTGCATCTTACAGGCTATGAC
CTGTCGATGGATGATATTCGCGACTTTCGTCAGCTCCACTCCCGCACGCCGGGTCATCCAGAATATGGTTATACGCCGGG
TGTTGAAACCACCACCGGCCCGTTGGGGCAGGGCGTAGCTAATGCGGTGGGGATGGCGATTGCGGAGAAGGCGCTGGCGG
CAGAATTTAACAAACCGGGCTTTAATATTGTTGATCATCACACATGGCTATTTTTGGGTGATGGCTGCCTGATGGAAGGG
ATCTCCCACGAGGCCTGTGGTCTTGCAGGAACGCTGAAGCTCGGCAATCTGATTGCTATCTGGGACGATAACGGTATTTC
GATCGATGGTCACGTAGAAGGCAGGTTTGCTGAAGATACGGCTGCCCGTTTTCGTGCCTACGGCTGGCATGTCATTGAAG
GCGTTGATGGTCACGATCCAGAAGAGGTAGATGCTGCGGTGCGTGAAGCGAAAAGCGTCACGGATAAGCCCAGTCTGCTG
TGCTGCAAAACGATTATCGGTTTTGGTTCGCCAAATAAAGCCAACAGCCACGATTGCCACGGCTCGGCGCTGGGTGCAGA
TGAAGTGGCGCTGGTGCGTGAACGTCTGCAATGGTCGTATGCCCCTTTCGAAATTCCTGGTGAAATTTATGCCGAATGGG
ATGCGACCGAGAAAGGCGCGCAGGTTCAACAGGAATGGGATGCACTGTTTGCCGACTACGCGAAGCAGTGGCCGGAACTG
GCGGCGGAGTTTACTCGTCGCATGAAGGGTGATTTGCCTGCTGGCTGGGTCGAGAATATGCAGAAATATGTGCACGATCT
GCAATCCCATCCGGCGGCGCTGGCAACCCGTCAGGTTAGCCAGAAATGCCTCAATCACTTCGCAGATATGCTGCCGGAAC
TGATGGGCGGCTCGGCGGACTTGTCGCCCTCTAACCTGACTCGCCATCAGAAATCGGTTGATTTCACCGGAGAAAATCCG
GCGGGGAATTACATCTCCTATGGCGTGCGTGAATTTGGTATGTCGGCCATCATGAACGGTCTTGCGCTGCATGGTGGGTT
TATCCCTTACGGCGGTACTTTCCTGATGTTTATGGAATATGCGCGTAATGCACTGCGCATGGCGGCGCTGATGAAGATCC
GCTCGGTGTTTGTTTATACCCACGATACCATCGGCCTGGGCGAAGACGGCCCGACACATCAGCCCGTTGAGCAACTGGCG
TCGCTGCGTCTGACGCCGAATATGGAAACCTGGCGCGGTTGCGATCAGGTGGAAGTGGCGGTGGCCTGGCAACAGGCGAT
TGAGCGCAAAGATGGCCCAACGTCGTTGGTATTAACGCGCCAACCTCTGGCGCAACAGCCGCGCACCGCTGCACAGTTGG
CAGAGATTGCACGTGGTGGTTATGTACTGAGTGATTGTGACGGTCAGCCTGAGATGATCCTCATTTCTGCGGGTTCAGAG
ATTGAGTTAGTGGTCAGCGCCGCAAAAGCGTTAACGGAAGAAGGGCGTAAAGTTCGCGTGGTTTCTATGCCGTGTACCGA
GCGTTTCGATAATCAGGATGCGGCGTACAAAGAATCGGTATTGCCAAAAGCTGTACGCAAACGTCTGGCGGTAGAAGCCA
GCATCGCCGGATTCTGGGAACGCTACGTCGGCCTGGACGGTAAAGTGATCGGCATGACGAGCTTTGGTGAGTCTGCTCCG
GCGAATGTGTTGTTCAAACACTTTGGTTTTACGCCGGAAAATGTCCTGGCGCAGGCGCGTGAGCTACTGAACAGCTAA

Domains

Predicted by InterProScan.

Transketolase, pyrimidine binding domain (Transket_pyr)

(354-524)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-333)

Transketolase, C-terminal domain (Transketolase_C)

(540-655)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

46.889

99.098

0.465