Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NKE59_RS02825 Genome accession   NZ_CP099959
Coordinates   577256..578620 (+) Length   454 a.a.
NCBI ID   WP_353439423.1    Uniprot ID   A0AAU8A3Y4
Organism   Polynucleobacter sp. UK-FUSCHL-C3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 572256..583620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKE59_RS02800 (NKE59_02790) - 572700..573398 (+) 699 WP_353439418.1 ABC transporter ATP-binding protein -
  NKE59_RS02805 (NKE59_02795) - 573403..574215 (+) 813 WP_353439419.1 TatD family hydrolase -
  NKE59_RS02815 (NKE59_02805) - 574916..576757 (+) 1842 WP_353439889.1 potassium transporter Kup -
  NKE59_RS02820 (NKE59_02810) - 576754..577251 (+) 498 WP_353439421.1 hypothetical protein -
  NKE59_RS02825 (NKE59_02815) radA 577256..578620 (+) 1365 WP_353439423.1 DNA repair protein RadA Machinery gene
  NKE59_RS02830 (NKE59_02820) prmB 578627..579538 (-) 912 WP_353439424.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  NKE59_RS02835 (NKE59_02825) dapE 579574..580722 (-) 1149 WP_353439425.1 succinyl-diaminopimelate desuccinylase -
  NKE59_RS02840 (NKE59_02830) dapD 580740..581567 (-) 828 WP_353439426.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48058.50 Da        Isoelectric Point: 7.1020

>NTDB_id=702961 NKE59_RS02825 WP_353439423.1 577256..578620(+) (radA) [Polynucleobacter sp. UK-FUSCHL-C3]
MAKTKTLYVCQACGGSSPKWQGQCPACQAWNTLEESLSETSSGNRFQGLTNSIPRQKLVSINAEDSPRLPTGIEELDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAELSAKGVDVLYSSGEESAAQIALRAKRIVLDSPSLEVLAEIQLEKLLLSVE
AAQPQVLVVDSIQTLYSEAFSSAPGSVAQVRECAAQLTRLAKSSGICVLLVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTERGLKGVNNPSALFLSQHPETVPGSCVLVTQEGSRPLLVEIQALVDTAHIPNPR
RLAVGLEQARLAMLLAVLHRHAGIACFDQDVFLNAVGGVKISEPAADLAVLLAIASSIRNRALPKSLAVFGEVGLAGEIR
PCPRGQERLKEAAKLGFSVAIIPKANLPKTKIANLQVIAVERIDEAIRAANDLS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=702961 NKE59_RS02825 WP_353439423.1 577256..578620(+) (radA) [Polynucleobacter sp. UK-FUSCHL-C3]
GTGGCTAAGACTAAAACTCTCTATGTCTGCCAAGCCTGTGGTGGCAGTTCACCCAAATGGCAAGGGCAGTGTCCCGCTTG
CCAGGCTTGGAATACCTTAGAAGAATCGCTTTCCGAGACATCATCAGGTAATCGTTTCCAAGGGCTTACCAACTCCATAC
CGCGGCAAAAATTAGTGAGCATTAATGCTGAGGATTCTCCACGTTTACCAACCGGTATTGAAGAGCTTGATCGTGTTTTA
GGTGGCGGTTTAGTGCCTGGTGGAGTTGTTCTTTTGGGAGGCGACCCCGGTATTGGCAAGTCTACCTTGCTGCTACAAGC
CCTAGCAGAGCTGAGCGCAAAAGGAGTCGATGTGCTCTATAGCAGCGGTGAGGAATCTGCTGCCCAAATTGCATTGCGCG
CAAAGCGCATTGTGTTGGATTCGCCTTCGCTTGAAGTGCTTGCGGAGATCCAATTAGAGAAATTGTTACTTTCTGTGGAA
GCTGCACAGCCCCAAGTCTTGGTAGTGGATTCGATTCAAACTCTGTATTCAGAAGCGTTTTCATCGGCACCCGGATCGGT
TGCGCAGGTTCGGGAGTGCGCAGCACAGCTCACGCGCTTGGCTAAATCGAGTGGTATCTGTGTTTTGTTAGTTGGCCATG
TGACCAAAGATGGGCATCTAGCAGGCCCCAGGGTGCTGGAGCATATTGTTGATACTGTTTTGTATTTTGAGGGTGATACC
CATTCCTCGTTTCGCTTAGTACGGTCTATCAAAAACCGCTTTGGTGCGGTTAATGAGTTAGGTGTCTTTGCGATGACCGA
GCGCGGTCTGAAGGGTGTTAATAATCCATCGGCATTATTTTTGTCCCAACATCCAGAAACCGTTCCGGGCTCTTGTGTAC
TAGTGACCCAAGAGGGTAGTCGTCCCTTATTAGTAGAGATTCAGGCTTTGGTGGATACGGCGCATATCCCCAATCCACGG
CGCTTGGCAGTGGGCTTGGAGCAGGCACGTCTTGCGATGTTGTTAGCGGTCTTGCATCGTCATGCCGGTATTGCCTGTTT
TGATCAAGATGTGTTTTTAAATGCGGTGGGAGGCGTCAAGATTAGTGAGCCCGCTGCAGACCTCGCTGTTCTCTTGGCAA
TCGCCTCGTCGATCCGTAATCGGGCTTTGCCAAAAAGCTTAGCGGTTTTTGGTGAGGTGGGACTCGCCGGTGAGATTCGA
CCATGTCCCCGCGGTCAAGAGCGCCTCAAAGAAGCTGCCAAGTTGGGATTTTCGGTCGCTATTATTCCCAAGGCAAACTT
ACCCAAGACAAAGATTGCCAATTTACAAGTGATTGCGGTTGAGCGCATTGATGAAGCTATTCGTGCTGCGAATGATCTGA
GTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(358-450)

AAA domain (AAA_25)

(68-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.89

100

0.5
  radA Streptococcus mitis SK321

47.333

99.119

0.469
  radA Streptococcus mitis NCTC 12261

47.333

99.119

0.469
  radA Streptococcus pneumoniae TIGR4

47.111

99.119

0.467
  radA Streptococcus pneumoniae R6

47.111

99.119

0.467
  radA Streptococcus pneumoniae Rx1

47.111

99.119

0.467
  radA Streptococcus pneumoniae D39

47.111

99.119

0.467