Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PCP76_RS13670 Genome accession   NZ_CP115297
Coordinates   2937661..2939388 (-) Length   575 a.a.
NCBI ID   WP_073649808.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 135     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2932661..2944388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP76_RS13640 (PCP76_13620) - 2933521..2934600 (-) 1080 WP_073649804.1 type II secretion system protein GspK -
  PCP76_RS13645 (PCP76_13625) - 2934590..2935180 (-) 591 WP_073649805.1 PulJ/GspJ family protein -
  PCP76_RS13650 (PCP76_13630) - 2935177..2935602 (-) 426 WP_057389526.1 PulJ/GspJ family protein -
  PCP76_RS13655 (PCP76_13635) - 2935602..2936012 (-) 411 WP_073649806.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PCP76_RS13660 (PCP76_13640) gspG 2935981..2936415 (-) 435 WP_073649807.1 type II secretion system major pseudopilin GspG -
  PCP76_RS13665 (PCP76_13645) - 2936469..2937656 (-) 1188 WP_057389523.1 type II secretion system F family protein -
  PCP76_RS13670 (PCP76_13650) pilF 2937661..2939388 (-) 1728 WP_073649808.1 GspE/PulE family protein Machinery gene
  PCP76_RS13675 (PCP76_13655) - 2939378..2940181 (-) 804 WP_057389521.1 ABC transporter permease -
  PCP76_RS13680 (PCP76_13660) - 2940801..2941553 (-) 753 WP_058178398.1 class I SAM-dependent methyltransferase -
  PCP76_RS13685 (PCP76_13665) - 2941779..2942753 (+) 975 WP_073649809.1 quinone oxidoreductase family protein -
  PCP76_RS13690 (PCP76_13670) - 2942776..2943705 (+) 930 WP_019485838.1 LysR family transcriptional regulator -
  PCP76_RS13695 (PCP76_13675) - 2943853..2944200 (+) 348 Protein_2702 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63622.66 Da        Isoelectric Point: 5.1654

>NTDB_id=702808 PCP76_RS13670 WP_073649808.1 2937661..2939388(-) (pilF) [Pseudomonas aeruginosa strain 135]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGPELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGADL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRQAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLITVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLGAADLAA
TEAATARLFPGQAAQWHEARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=702808 PCP76_RS13670 WP_073649808.1 2937661..2939388(-) (pilF) [Pseudomonas aeruginosa strain 135]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCCGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAATTGCGGTTGCCGCTGGTGGGGGCCGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTTCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCCGTGCTGTGTCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCAAGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTGGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGTCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAACTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCGGGGGTGCATGGTGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACGACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCACCGTGGAAGATCCCGTGGAATACCAGGTGCCCAATATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGCGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCATTGCGCGGAACCTTGCGAGCCAACCCTTGGTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCACGAGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAGCAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

42.049

98.435

0.414
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.801

98.609

0.383