Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   NI386_RS09755 Genome accession   NZ_CP099924
Coordinates   349661..350239 (+) Length   192 a.a.
NCBI ID   WP_015297396.1    Uniprot ID   A0A243VIJ8
Organism   Vibrio parahaemolyticus strain Isc7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344661..355239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI386_RS09740 (NI386_09715) oxyR 344984..345892 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  NI386_RS09745 (NI386_09720) - 345948..348518 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  NI386_RS09750 (NI386_09725) pilM 348661..349677 (+) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  NI386_RS09755 (NI386_09730) pilN 349661..350239 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  NI386_RS09760 (NI386_09735) pilO 350232..350825 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI386_RS09765 (NI386_09740) pilP 350815..351330 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  NI386_RS09770 (NI386_09745) pilQ 351362..353110 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  NI386_RS09775 (NI386_09750) aroK 353299..353817 (+) 519 WP_005381319.1 shikimate kinase AroK -
  NI386_RS09780 (NI386_09755) aroB 353849..354949 (+) 1101 WP_308390555.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.95 Da        Isoelectric Point: 6.9944

>NTDB_id=702627 NI386_RS09755 WP_015297396.1 349661..350239(+) (pilN) [Vibrio parahaemolyticus strain Isc7]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=702627 NI386_RS09755 WP_015297396.1 349661..350239(+) (pilN) [Vibrio parahaemolyticus strain Isc7]
GTGCTGTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGCGTCATGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTACCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCTGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCACGGCTAGCAACCATGCTCGATAACATGGAACGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTTGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTTAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243VIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594