Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NHU86_RS01955 Genome accession   NZ_CP099742
Coordinates   405711..407150 (+) Length   479 a.a.
NCBI ID   WP_039040474.1    Uniprot ID   -
Organism   Aeromonas caviae strain OT55     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 400711..412150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHU86_RS01925 (NHU86_01920) mlaF 401289..402089 (-) 801 WP_029314846.1 phospholipid ABC transporter ATP-binding protein MlaF -
  NHU86_RS01930 (NHU86_01925) - 402296..403291 (+) 996 WP_029314845.1 KpsF/GutQ family sugar-phosphate isomerase -
  NHU86_RS01935 (NHU86_01930) kdsC 403291..403845 (+) 555 WP_042864828.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NHU86_RS01940 (NHU86_01935) lptC 403842..404402 (+) 561 WP_039040473.1 LPS export ABC transporter periplasmic protein LptC -
  NHU86_RS01945 (NHU86_01940) lptA 404389..404907 (+) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  NHU86_RS01950 (NHU86_01945) lptB 404920..405645 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  NHU86_RS01955 (NHU86_01950) rpoN/rpoN1 405711..407150 (+) 1440 WP_039040474.1 RNA polymerase factor sigma-54 Machinery gene
  NHU86_RS01960 (NHU86_01955) hpf 407171..407458 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  NHU86_RS01965 (NHU86_01960) ptsN 407461..407907 (+) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NHU86_RS01970 (NHU86_01965) rapZ 407948..408814 (+) 867 WP_010675869.1 RNase adapter RapZ -
  NHU86_RS01975 (NHU86_01970) npr 408835..409107 (+) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  NHU86_RS01980 (NHU86_01975) - 409123..411027 (-) 1905 WP_236758557.1 transcription antiterminator -
  NHU86_RS01985 (NHU86_01980) - 411131..411871 (-) 741 WP_113069534.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53761.57 Da        Isoelectric Point: 4.3728

>NTDB_id=701435 NHU86_RS01955 WP_039040474.1 405711..407150(+) (rpoN/rpoN1) [Aeromonas caviae strain OT55]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQTETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=701435 NHU86_RS01955 WP_039040474.1 405711..407150(+) (rpoN/rpoN1) [Aeromonas caviae strain OT55]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGACAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACGGCCCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAGGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCTGCCATCGCGCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCGGACCTGCCCT
GGCTCGACGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTTGAACTCAATCCGG
ATTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCTCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482