Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NHU85_RS13555 Genome accession   NZ_CP099739
Coordinates   2970605..2972074 (+) Length   489 a.a.
NCBI ID   WP_005281701.1    Uniprot ID   D4F169
Organism   Edwardsiella tarda strain GT88     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2965605..2977074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHU85_RS13525 (NHU85_13515) - 2966035..2967024 (+) 990 WP_005281708.1 calcium/sodium antiporter -
  NHU85_RS13530 (NHU85_13520) kdsD 2967077..2968063 (+) 987 WP_302528557.1 arabinose-5-phosphate isomerase KdsD -
  NHU85_RS13535 (NHU85_13525) kdsC 2968079..2968645 (+) 567 WP_035604658.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NHU85_RS13540 (NHU85_13530) lptC 2968642..2969214 (+) 573 WP_035604656.1 LPS export ABC transporter periplasmic protein LptC -
  NHU85_RS13545 (NHU85_13535) lptA 2969189..2969746 (+) 558 WP_005281704.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NHU85_RS13550 (NHU85_13540) lptB 2969753..2970478 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  NHU85_RS13555 (NHU85_13545) rpoN/rpoN1 2970605..2972074 (+) 1470 WP_005281701.1 RNA polymerase factor sigma-54 Machinery gene
  NHU85_RS13560 (NHU85_13550) hpf 2972096..2972383 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  NHU85_RS13565 (NHU85_13555) ptsN 2972518..2972964 (+) 447 WP_005297331.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NHU85_RS13570 (NHU85_13560) rapZ 2973072..2973923 (+) 852 WP_005281696.1 RNase adapter RapZ -
  NHU85_RS13575 (NHU85_13565) npr 2973931..2974203 (+) 273 WP_005281695.1 PTS phosphocarrier protein NPr -
  NHU85_RS13580 (NHU85_13570) rnk 2974380..2974790 (+) 411 WP_035597411.1 nucleoside diphosphate kinase regulator -
  NHU85_RS13585 (NHU85_13575) - 2974826..2975329 (-) 504 WP_078000406.1 cytochrome b/b6 domain-containing protein -
  NHU85_RS13590 (NHU85_13580) cybC 2975335..2975721 (-) 387 WP_035604649.1 cytochrome b562 -
  NHU85_RS13595 (NHU85_13585) deoD 2975935..2976660 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55075.91 Da        Isoelectric Point: 4.4068

>NTDB_id=701428 NHU85_RS13555 WP_005281701.1 2970605..2972074(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT88]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVRENTQEDVVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLEEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQSLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=701428 NHU85_RS13555 WP_005281701.1 2970605..2972074(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT88]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGCTGGCCATGACGCCCCAACTGCAACAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTGGAGTTACAGCAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCGCGAGAATACACAGGAGGATGTCGTCACACGCGATGATGCCGATCCACTGGAT
AGCCGCGAGGCGTTGGAGCAACGTGAGATGCCGGACGAGCTGCCGCTGGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCAGGCACGGGAACAGATTATCATGACGACGAGTTACCCGTGTTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCTACCGCCATCGTCGATGCCGTC
GATGATACCGGCTATCTCAGCGCTTCGTTGGAGGAGATCCTCGAGAGTCTAGGGGACGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGATCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACGCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATCCAGAG
CCTCGACCCGCGGCCAGGACTCTCCATCAATACCGGCGAGTCTGACTACGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAGCGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAACCAACATTACGCCGCGCTGGGCACT
CAGGCGCGTAACGACAGCGACAGCCAGTTCATTCGTAGCAATCTGCAAGAAGCGAAGTGGCTGATCAAGAGCTTAGAGAG
CCGTAACGATACCCTGCTGAAGGTATCGCGTTGCATCGTAGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTACTGGCTGATATCGCTCAGCAAGTCGAAATGCACGAATCGACGATCTCACGTGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGTGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCCTCCACGGCCATCCGGGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAACCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCGGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4F169

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481