Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NHH62_RS21145 Genome accession   NZ_CP099647
Coordinates   418317..419834 (-) Length   505 a.a.
NCBI ID   WP_028199090.1    Uniprot ID   -
Organism   Paraburkholderia fungorum strain OTU2SAUBB1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413317..424834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHH62_RS21120 (NHH62_21125) - 414171..414803 (-) 633 WP_028199086.1 LON peptidase substrate-binding domain-containing protein -
  NHH62_RS21125 (NHH62_21130) rapZ 414843..415736 (-) 894 WP_028199087.1 RNase adapter RapZ -
  NHH62_RS21130 (NHH62_21135) hprK 415843..416811 (-) 969 WP_007179412.1 HPr(Ser) kinase/phosphatase -
  NHH62_RS21135 (NHH62_21140) - 416971..417486 (-) 516 WP_081833202.1 PTS sugar transporter subunit IIA -
  NHH62_RS21140 (NHH62_21145) raiA 417790..418146 (-) 357 WP_028199089.1 ribosome-associated translation inhibitor RaiA -
  NHH62_RS21145 (NHH62_21150) rpoN/rpoN1 418317..419834 (-) 1518 WP_028199090.1 RNA polymerase factor sigma-54 Machinery gene
  NHH62_RS21150 (NHH62_21155) lptB 420020..420805 (-) 786 WP_028199091.1 LPS export ABC transporter ATP-binding protein -
  NHH62_RS21155 (NHH62_21160) lptA 420802..421473 (-) 672 WP_028199092.1 lipopolysaccharide transport periplasmic protein LptA -
  NHH62_RS21160 (NHH62_21165) lptC 421494..422102 (-) 609 WP_030099920.1 LPS export ABC transporter periplasmic protein LptC -
  NHH62_RS21165 (NHH62_21170) - 422099..422659 (-) 561 WP_030099919.1 HAD family hydrolase -
  NHH62_RS21170 (NHH62_21175) kdsD 422659..423642 (-) 984 WP_030099918.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55583.70 Da        Isoelectric Point: 5.0733

>NTDB_id=701235 NHH62_RS21145 WP_028199090.1 418317..419834(-) (rpoN/rpoN1) [Paraburkholderia fungorum strain OTU2SAUBB1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAISQNPLLENEDDWIASPLRVAADGSLIAQAPNSSPPSDQM
GGNTSSSSTSSSERAENGEPQGVDEYNGLASDGNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QASQRDRALITFLIESLDDDGYLAATLEEVLADLPDELEVDLDEMNAALALLHSFDPAGVGARSASECLKLQLLRLEQSP
TRTLALDIVAHHLELLAARDFTRLRKHLKANDDDLREAHILIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=701235 NHH62_RS21145 WP_028199090.1 418317..419834(-) (rpoN/rpoN1) [Paraburkholderia fungorum strain OTU2SAUBB1]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACCCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCCACGCTCGAACTGCAGCAGGAAGTCGCAATGGCGATCTCGCAGAATCCGCTCCTCGAGAATGAGGACGACTGGA
TCGCGAGCCCCCTGCGCGTGGCGGCCGACGGTTCCCTGATCGCCCAGGCGCCCAATTCTTCGCCACCGTCCGACCAGATG
GGCGGCAATACGTCGTCCTCGTCCACCAGCAGCAGCGAGCGTGCCGAGAATGGCGAACCGCAGGGTGTGGACGAATACAA
CGGCCTCGCGAGCGACGGCAACGGCGATGCGTCGCAATGGAATCTCGACGATTACGGCCGCTCCGGCAATGCGTCGGACG
ACGACGATCTGCCGCCGCTGCAAATCCACGAATCGAGCACCTCGCTGCGCGATCACCTGATGGCGCAACTTCGCGTCACC
CAGGCAAGCCAGCGCGACCGCGCGCTGATCACCTTCCTGATCGAATCGCTCGACGACGACGGCTACCTTGCCGCCACGCT
CGAAGAAGTGCTGGCCGATCTGCCCGATGAGCTCGAAGTCGATCTCGACGAAATGAATGCCGCGCTGGCGTTGCTGCACA
GCTTCGACCCGGCCGGGGTCGGTGCGCGCTCCGCGTCGGAATGTCTCAAGCTGCAATTGCTGCGGCTCGAGCAGTCGCCT
ACCCGCACACTTGCGCTGGACATCGTCGCCCATCATCTGGAACTGCTCGCGGCACGCGATTTCACGCGCCTGCGCAAGCA
TTTGAAGGCAAACGACGACGATCTGCGCGAAGCGCATATTCTGATCCGCTCGCTCGAGCCGTTTCCGGGCGCTGCGTACG
GCAAGGCCGAGGCCGACTACGTGGTGCCGGACATCATGGTGCGCAAAACGGCACAGGGATGGCAGGCGGAGCTCAATCCG
GAAGTGGTGCCGAAGCTGCGTATCAACCATCTATACGCGAATATTCTGCGCAATAACCGTGGCGATCCGGGCAGTGGATC
GTTGCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGAGACGATCCTGCGGGTCGCCC
AGGCTATTGTCGAGCGTCAAAAGAGCTTTTTTGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGAGAAATTGCT
GATACGCTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTCGGATCACACGTTTCGACTGACACGGGCGGCGCGGCCTCATCTACGGCCATTCGCGCGCTCATCA
AGCAACTGATAGGAGCGGAAAACCCGAAATCTCCTCTTTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGTACCGTTGCGAAGTATCGCGAAGCGCTGAAGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.602

100

0.675