Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NFE55_RS02930 Genome accession   NZ_CP099623
Coordinates   639042..640559 (-) Length   505 a.a.
NCBI ID   WP_046566900.1    Uniprot ID   A0AAP5Q7B5
Organism   Paraburkholderia fungorum strain OTU2BAGNBA1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 634042..645559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFE55_RS02905 (NFE55_02905) - 634895..635527 (-) 633 WP_028199086.1 LON peptidase substrate-binding domain-containing protein -
  NFE55_RS02910 (NFE55_02910) rapZ 635567..636460 (-) 894 WP_028199087.1 RNase adapter RapZ -
  NFE55_RS02915 (NFE55_02915) hprK 636566..637534 (-) 969 WP_007179412.1 HPr(Ser) kinase/phosphatase -
  NFE55_RS02920 (NFE55_02920) - 637694..638209 (-) 516 WP_081833202.1 PTS sugar transporter subunit IIA -
  NFE55_RS02925 (NFE55_02925) raiA 638515..638871 (-) 357 WP_028199089.1 ribosome-associated translation inhibitor RaiA -
  NFE55_RS02930 (NFE55_02930) rpoN/rpoN1 639042..640559 (-) 1518 WP_046566900.1 RNA polymerase factor sigma-54 Machinery gene
  NFE55_RS02935 (NFE55_02935) lptB 640745..641530 (-) 786 WP_028199091.1 LPS export ABC transporter ATP-binding protein -
  NFE55_RS02940 (NFE55_02940) lptA 641527..642198 (-) 672 WP_028199092.1 lipopolysaccharide transport periplasmic protein LptA -
  NFE55_RS02945 (NFE55_02945) lptC 642219..642827 (-) 609 WP_144193147.1 LPS export ABC transporter periplasmic protein LptC -
  NFE55_RS02950 (NFE55_02950) - 642824..643384 (-) 561 WP_030099919.1 HAD family hydrolase -
  NFE55_RS02955 (NFE55_02955) kdsD 643384..644367 (-) 984 WP_028199095.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55569.67 Da        Isoelectric Point: 5.0706

>NTDB_id=701126 NFE55_RS02930 WP_046566900.1 639042..640559(-) (rpoN/rpoN1) [Paraburkholderia fungorum strain OTU2BAGNBA1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAISQNPLLENEDDWIASPLRVAADGSLIAQAPNSSPPSDQM
GGNTSSSSTSSSERAENGEPQGVDEYNGLASDGNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QASQRDRALITFLIESLDDDGYLAATLEEVLADLPDELEVDLDEMNAALALLHSFDPAGVGARSASECLKLQLLRLEQSP
TRTLALDIVAHHLELLAARDFTRLRKHLKANDDDLRDAHILIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=701126 NFE55_RS02930 WP_046566900.1 639042..640559(-) (rpoN/rpoN1) [Paraburkholderia fungorum strain OTU2BAGNBA1]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACCCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCCACGCTCGAACTGCAGCAGGAAGTCGCAATGGCGATCTCGCAGAATCCGCTCCTCGAGAATGAGGACGACTGGA
TCGCGAGCCCCCTGCGCGTGGCGGCCGACGGTTCCCTGATCGCCCAGGCGCCCAATTCCTCGCCACCGTCGGACCAGATG
GGCGGCAATACGTCGTCTTCGTCCACCAGCAGCAGCGAGCGTGCCGAGAACGGCGAACCGCAGGGTGTGGACGAATACAA
CGGCCTTGCGAGCGATGGCAACGGCGATGCGTCGCAATGGAATCTCGACGATTACGGCCGCTCCGGTAATGCCTCGGACG
ACGACGATCTGCCGCCGCTGCAAATCCACGAATCGAGCACCTCGCTGCGCGATCACCTGATGGCGCAACTCCGCGTCACC
CAGGCGAGCCAACGCGACCGCGCGCTGATCACCTTCCTGATCGAATCGCTCGACGACGACGGCTATCTTGCCGCCACGCT
CGAAGAGGTGCTGGCCGATCTGCCCGATGAGCTCGAAGTCGATCTCGACGAAATGAATGCCGCGCTGGCGTTGCTGCATA
GCTTCGATCCGGCCGGGGTCGGTGCGCGCTCCGCGTCTGAATGTCTGAAGCTGCAATTGCTGCGGCTCGAGCAGTCGCCT
ACCCGCACACTTGCGCTCGACATCGTCGCCCATCATCTGGAACTGCTCGCGGCACGCGATTTCACGCGCCTGCGCAAGCA
TCTGAAGGCGAACGACGACGATCTGCGCGATGCGCATATTCTGATCCGCTCGCTCGAGCCGTTTCCGGGCGCTGCGTACG
GCAAGGCCGAGGCCGACTACGTGGTGCCGGACATCATGGTGCGCAAAACGGCACAGGGATGGCAGGCGGAGCTCAATCCG
GAAGTGGTGCCGAAGCTGCGTATCAACCATCTATACGCGAATATTCTGCGCAATAACCGTGGCGATCCGGGCAGTGGATC
GTTGCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGAGACGATCCTGCGGGTCGCCC
AGGCTATTGTCGAGCGTCAAAAGAGCTTTTTTGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGAGAAATTGCT
GATACGCTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTCGGATCACACGTTTCGACTGACACGGGCGGCGCGGCCTCATCCACGGCCATTCGCGCGCTCATCA
AGCAACTGATAGGAGCGGAAAACCCGAAATCTCCTCTTTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGTACCGTTGCGAAGTATCGCGAAGCGCTGAAGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.602

100

0.675