Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NHB35_RS10050 Genome accession   NZ_CP099572
Coordinates   1937707..1938597 (+) Length   296 a.a.
NCBI ID   WP_353432222.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-UH23A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1932707..1943597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHB35_RS10020 (NHB35_10010) pth 1932919..1933509 (+) 591 WP_353432214.1 aminoacyl-tRNA hydrolase -
  NHB35_RS10025 (NHB35_10015) - 1933513..1933779 (-) 267 WP_353432216.1 YfhL family 4Fe-4S dicluster ferredoxin -
  NHB35_RS10030 (NHB35_10020) coaD 1933801..1934298 (-) 498 WP_353432217.1 pantetheine-phosphate adenylyltransferase -
  NHB35_RS10035 (NHB35_10025) rsmD 1934335..1934931 (-) 597 WP_353432219.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NHB35_RS10040 (NHB35_10030) - 1934942..1936279 (-) 1338 WP_353432220.1 pitrilysin family protein -
  NHB35_RS10045 (NHB35_10035) - 1936276..1937643 (-) 1368 WP_353432221.1 pitrilysin family protein -
  NHB35_RS10050 (NHB35_10040) pilA 1937707..1938597 (+) 891 WP_353432222.1 signal recognition particle-docking protein FtsY Machinery gene
  NHB35_RS10055 (NHB35_10045) rpoH 1938693..1939616 (+) 924 WP_353432223.1 RNA polymerase sigma factor RpoH -
  NHB35_RS10060 (NHB35_10050) - 1939647..1940237 (-) 591 WP_353432224.1 SCO family protein -
  NHB35_RS10065 (NHB35_10055) cyoE 1940249..1941142 (-) 894 WP_353432225.1 heme o synthase -
  NHB35_RS10070 (NHB35_10060) - 1941139..1942242 (-) 1104 WP_353432227.1 COX15/CtaA family protein -
  NHB35_RS10075 (NHB35_10065) - 1942242..1942898 (-) 657 WP_353432228.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31314.90 Da        Isoelectric Point: 7.9809

>NTDB_id=700739 NHB35_RS10050 WP_353432222.1 1937707..1938597(+) (pilA) [Polynucleobacter sp. MWH-UH23A]
MFGLRKTLGSLFKSSKLDDAWFDHLEESLIQSDVGLPTTEQLMAKLRKAAKSEKASSAQELQALLVQEVTSLLQSLEPSP
NPLFTSTSSHIPEVWLVVGVNGAGKTTTIGKLCKLFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQEGGDAAA
VAHDAIHSAVSRKNDVLIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTA
IIVTKLDGTAKGGVICALAQTLKDGPKPAILALGKGEGIDDLAPFKAGQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=700739 NHB35_RS10050 WP_353432222.1 1937707..1938597(+) (pilA) [Polynucleobacter sp. MWH-UH23A]
ATGTTTGGATTACGTAAAACCCTAGGATCTCTATTTAAATCAAGCAAATTAGATGATGCTTGGTTTGATCATTTGGAAGA
ATCACTCATTCAGAGTGATGTGGGTTTGCCTACGACCGAACAATTAATGGCTAAGCTGCGCAAAGCCGCTAAATCGGAAA
AAGCATCTAGCGCACAAGAGCTACAAGCATTGCTAGTTCAAGAGGTTACAAGCTTATTACAAAGTCTTGAGCCTAGCCCG
AATCCTTTATTTACAAGCACTTCAAGCCATATTCCTGAAGTATGGCTAGTAGTTGGCGTCAATGGCGCAGGCAAAACAAC
CACTATTGGAAAGCTTTGTAAGCTCTTTCAATCTCAAGGCAAATCGGTGCTACTGGCAGCAGGTGATACCTTTCGAGCTG
CTGCACGAAATCAATTGCAAGAATGGGGTGGACGCAACCAAGTTGACGTTATTACTCAAGAGGGCGGGGATGCAGCAGCT
GTTGCACACGATGCAATTCACTCTGCAGTCTCTCGCAAAAATGATGTTCTGATTATCGACACTGCAGGTCGCCTTGCCAC
TCAAGACCATCTGATGGAAGAGCTCAAGAAAGTCAAAAGAGTGATTGGCAAAGCCCTTCCTGGAGCACCTCACCATACCC
TTCTAGTTCTAGATGGCAATACCGGACAAAATGGCTTAAGCCAAGTAAAGGCATTTCATGCTGCTCTGGGCCTTACTGCA
ATCATCGTTACCAAGCTTGATGGCACCGCTAAAGGGGGTGTTATTTGCGCCCTAGCCCAAACCCTCAAAGATGGCCCCAA
ACCAGCAATCTTGGCACTCGGTAAGGGTGAGGGAATTGATGATTTAGCCCCATTCAAAGCAGGGCAATATTCCTCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(92-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.877

98.986

0.514