Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NHB35_RS07815 Genome accession   NZ_CP099572
Coordinates   1497992..1499356 (-) Length   454 a.a.
NCBI ID   WP_353431815.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-UH23A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1492992..1504356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHB35_RS07800 (NHB35_07790) dapD 1495039..1495866 (+) 828 WP_215389188.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  NHB35_RS07805 (NHB35_07795) dapE 1495888..1497039 (+) 1152 WP_353431813.1 succinyl-diaminopimelate desuccinylase -
  NHB35_RS07810 (NHB35_07800) prmB 1497084..1497980 (+) 897 WP_353431814.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  NHB35_RS07815 (NHB35_07805) radA 1497992..1499356 (-) 1365 WP_353431815.1 DNA repair protein RadA Machinery gene
  NHB35_RS07820 (NHB35_07810) - 1499393..1499890 (-) 498 WP_353431816.1 hypothetical protein -
  NHB35_RS07825 (NHB35_07815) - 1499899..1501740 (-) 1842 WP_353433425.1 potassium transporter Kup -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48265.07 Da        Isoelectric Point: 7.6031

>NTDB_id=700734 NHB35_RS07815 WP_353431815.1 1497992..1499356(-) (radA) [Polynucleobacter sp. MWH-UH23A]
MAKVKTVYICQSCGGTSAKWQGQCPSCQSWNTLEEGLPETTSNSRFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSAVGMNVLYSSGEESAAQIALRAKRISLNAPQLEVLAEIQLEKLISIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKASGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLIRSIKNRFGAVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHVPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKTKIAGLRVIPVERIDQAIAAAAELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=700734 NHB35_RS07815 WP_353431815.1 1497992..1499356(-) (radA) [Polynucleobacter sp. MWH-UH23A]
TTGGCTAAAGTAAAAACGGTTTATATCTGTCAGTCCTGTGGAGGCACTTCTGCGAAGTGGCAAGGGCAATGCCCATCTTG
TCAGTCTTGGAACACTCTAGAAGAGGGTTTGCCAGAGACGACATCTAATTCTCGATTTCAGGGCTTGGCGCAATCTTTGC
CCCGACAAAAGCTCTCGGCCATTACGGCGGAAGATTTGCCAAGATTTAGCACAGGTGTAGAAGAGTTTGATCGCGTATTG
GGTGGCGGATTGGTTCCTGGTGGCGTTGTGCTCCTGGGCGGTGATCCTGGTATTGGTAAATCTACCCTGCTGTTGCAAGC
TCTCGCGGAGATGAGTGCTGTTGGTATGAATGTTCTCTACAGTAGCGGCGAAGAATCCGCCGCACAAATTGCCTTACGCG
CGAAGCGCATCTCATTAAATGCTCCGCAGTTAGAAGTCCTGGCCGAAATTCAGTTGGAGAAACTGATTTCGATTATGGAT
ACCGTTAAGCCACAAGTATTAGTGGTGGATTCCATACAAACTTTGTATTCTGAAGTATTAAGCTCGGCTCCGGGATCGGT
GGCGCAAGTGCGCGAGTGCGCCGCTCAATTAACCAGGGCGGCTAAGGCTAGTGGCATTTGTGTGTTAATGGTGGGGCATG
TCACCAAAGATGGTCATTTAGCAGGTCCTCGAGTTCTCGAGCATATTGTCGATACCGTGTTGTACTTTGAGGGCGATACC
CATTCTTCTTTTCGCTTAATTCGTTCTATCAAAAATCGCTTTGGTGCAGTGAACGAATTGGGCGTGTTTGCGATGACTGA
AAAAGGTCTGCGCGGAGTGACAAATCCATCAGCTATTTTCTTGTCGCAACACGAGCAAATGGTTCCGGGAGCGTGTGTAC
TGGTAACTCAAGAGGGTAGCAGACCCCTGTTAGTTGAGATTCAGGCGCTTGTGGATACTGCGCATGTACCAAATCCTCGA
CGTTTAGCCGTTGGTTTAGAGCAGGCGCGCTTAGCTATGTTGCTGGCGGTTTTGCACCGTCATGCAGGTGTGGCTTGTTT
TGATCAGGATGTGTTCTTAAATGCAGTTGGTGGCGTGAAGATTTCAGAGCCAGCTGCTGACTTGGCTGTACTGCTTGCGA
TTCAATCATCTATTCGTAATCGCGCGCTACCAAAAGAGTTGATTGTGTTTGGCGAAGTGGGTTTAGCGGGAGAAATCCGC
CCATGTCCACGCGGGCAAGAGCGTCTAAAGGAAGCAGCAAAACTGGGCTTTACTGTTGCCATCATTCCGAAGGCCAATAT
GCCAAAGACAAAAATTGCAGGCTTGAGAGTGATTCCTGTGGAGCGTATTGATCAGGCGATCGCTGCAGCTGCAGAATTAA
GTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.336

99.559

0.491
  radA Streptococcus mitis NCTC 12261

47.319

94.493

0.447
  radA Streptococcus pneumoniae Rx1

47.319

94.493

0.447
  radA Streptococcus pneumoniae D39

47.319

94.493

0.447
  radA Streptococcus pneumoniae R6

47.319

94.493

0.447
  radA Streptococcus pneumoniae TIGR4

47.319

94.493

0.447
  radA Streptococcus mitis SK321

47.319

94.493

0.447