Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NHB34_RS09270 Genome accession   NZ_CP099571
Coordinates   1824404..1825294 (+) Length   296 a.a.
NCBI ID   WP_353427350.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-UH19D     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1819404..1830294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHB34_RS09240 (NHB34_09235) pth 1819614..1820204 (+) 591 WP_353427345.1 aminoacyl-tRNA hydrolase -
  NHB34_RS09245 (NHB34_09240) - 1820217..1820477 (-) 261 WP_353427346.1 YfhL family 4Fe-4S dicluster ferredoxin -
  NHB34_RS09250 (NHB34_09245) coaD 1820504..1820995 (-) 492 WP_353427347.1 pantetheine-phosphate adenylyltransferase -
  NHB34_RS09255 (NHB34_09250) rsmD 1821032..1821646 (-) 615 WP_353428589.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NHB34_RS09260 (NHB34_09255) - 1821639..1822976 (-) 1338 WP_353427348.1 pitrilysin family protein -
  NHB34_RS09265 (NHB34_09260) - 1822973..1824340 (-) 1368 WP_353427349.1 pitrilysin family protein -
  NHB34_RS09270 (NHB34_09265) pilA 1824404..1825294 (+) 891 WP_353427350.1 signal recognition particle-docking protein FtsY Machinery gene
  NHB34_RS09275 (NHB34_09270) rpoH 1825389..1826318 (+) 930 WP_353427351.1 RNA polymerase sigma factor RpoH -
  NHB34_RS09280 (NHB34_09275) - 1826337..1826927 (-) 591 WP_353427352.1 SCO family protein -
  NHB34_RS09285 (NHB34_09280) cyoE 1826938..1827831 (-) 894 WP_353427353.1 heme o synthase -
  NHB34_RS09290 (NHB34_09285) - 1827828..1828931 (-) 1104 WP_353427354.1 COX15/CtaA family protein -
  NHB34_RS09295 (NHB34_09290) - 1828931..1829587 (-) 657 WP_353427355.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31284.79 Da        Isoelectric Point: 6.9752

>NTDB_id=700729 NHB34_RS09270 WP_353427350.1 1824404..1825294(+) (pilA) [Polynucleobacter sp. MWH-UH19D]
MFGLRKTLGSLFKSSKADDAWFDHLEESLIQSDVGLPTTEQLMVKLRKAAASENASSAEELQALLIQEVANLLRSLEPNP
NPLLINTSSHTPEVWLVVGVNGAGKTTTIGKLCRLFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQEGGDAAA
VAHDAIHSAISRKADILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTA
IIVTKLDGTAKGGVICALAHTLKEGPKPAILALGKGEGIDDLAPFSAGQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=700729 NHB34_RS09270 WP_353427350.1 1824404..1825294(+) (pilA) [Polynucleobacter sp. MWH-UH19D]
ATGTTTGGATTACGTAAAACCCTAGGATCTCTATTTAAATCAAGCAAAGCAGATGATGCTTGGTTTGATCATTTAGAAGA
ATCACTGATTCAAAGTGATGTGGGTTTGCCCACGACTGAACAATTGATGGTTAAGCTACGTAAAGCAGCTGCATCAGAAA
ATGCTTCAAGCGCGGAAGAGCTCCAGGCTCTGCTGATTCAAGAGGTCGCCAACTTATTACGAAGCTTAGAACCTAACCCA
AACCCCCTGCTGATTAATACCTCAAGTCATACCCCTGAAGTATGGTTGGTTGTCGGCGTAAATGGCGCAGGCAAAACCAC
CACCATCGGCAAACTCTGCAGACTCTTTCAATCTCAAGGTAAGTCAGTTTTACTGGCTGCAGGCGACACCTTTAGAGCGG
CGGCACGCAATCAATTGCAAGAATGGGGTGGGCGCAATCAAGTTGATGTTATTACTCAAGAGGGTGGAGACGCCGCTGCA
GTCGCTCACGATGCAATTCACTCTGCAATCTCTCGCAAAGCTGACATTCTGATTATTGATACTGCAGGTCGTCTTGCCAC
CCAGGATCACCTCATGGAAGAACTCAAAAAAGTAAAAAGGGTTATCGGCAAAGCCCTTCCAGGGGCGCCCCATCACACCC
TATTAGTCTTAGATGGCAACACCGGGCAAAATGGCCTAAGCCAAGTAAAAGCCTTTCATGCAGCTTTGGGTCTTACTGCA
ATCATTGTGACAAAGCTCGATGGCACAGCCAAAGGGGGAGTTATATGCGCTCTTGCCCACACCCTAAAGGAGGGTCCCAA
ACCAGCCATTTTGGCCCTTGGCAAAGGGGAGGGAATTGATGATTTAGCCCCATTCTCAGCAGGGCAATATTCCTCAGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(92-295)

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

51.536

98.986

0.51