Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NHB34_RS07195 Genome accession   NZ_CP099571
Coordinates   1421290..1422654 (-) Length   454 a.a.
NCBI ID   WP_353426968.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-UH19D     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1416290..1427654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHB34_RS07180 (NHB34_07180) dapD 1418330..1419157 (+) 828 WP_353426965.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  NHB34_RS07185 (NHB34_07185) dapE 1419177..1420328 (+) 1152 WP_353426966.1 succinyl-diaminopimelate desuccinylase -
  NHB34_RS07190 (NHB34_07190) prmB 1420371..1421267 (+) 897 WP_353426967.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  NHB34_RS07195 (NHB34_07195) radA 1421290..1422654 (-) 1365 WP_353426968.1 DNA repair protein RadA Machinery gene
  NHB34_RS07200 (NHB34_07200) - 1422684..1423121 (-) 438 WP_353426969.1 hypothetical protein -
  NHB34_RS07205 (NHB34_07205) - 1423190..1425031 (-) 1842 WP_353428569.1 potassium transporter Kup -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48207.05 Da        Isoelectric Point: 7.3470

>NTDB_id=700724 NHB34_RS07195 WP_353426968.1 1421290..1422654(-) (radA) [Polynucleobacter sp. MWH-UH19D]
MAKVKTIYICQSCGGTAAKWQGQCPSCQSWNTMEEGLPESSSNSRFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVQGGVVLLGGDPGIGKSTLLLQALAEMSAAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLIAIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKASGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLIRSIKNRFGAVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHIPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKAKIPGLKVIPVERIDQAIAAAAELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=700724 NHB34_RS07195 WP_353426968.1 1421290..1422654(-) (radA) [Polynucleobacter sp. MWH-UH19D]
TTGGCCAAAGTAAAAACAATCTATATCTGTCAGTCTTGCGGCGGTACTGCAGCAAAGTGGCAAGGTCAATGCCCATCTTG
CCAGTCATGGAACACGATGGAAGAGGGGCTCCCCGAGTCCTCATCTAATTCTCGTTTTCAGGGTTTGGCGCAATCGTTGC
CAAGACAAAAGCTTTCCGCTATCACGGCGGAAGATTTGCCAAGATTTAGTACTGGTGTAGAAGAGTTTGACCGCGTATTG
GGTGGCGGATTAGTTCAGGGTGGTGTTGTGCTGCTTGGCGGAGATCCGGGCATCGGTAAATCTACTTTGTTATTGCAAGC
ACTAGCTGAGATGAGTGCTGCAGGTATGAATGTTCTTTATAGCAGTGGTGAGGAATCAGCAGCGCAAATTGCCTTGCGTG
CCAAAAGAATTGCACTAGATGCGCCCCAATTAGAAGTCTTGGCAGAAATTCAGCTAGAAAAGCTGATTGCGATTATGGAT
ACGGTGAAGCCACAAGTATTGGTTGTGGACTCAATTCAGACTTTGTACTCCGAAGTATTAAGTTCTGCGCCTGGATCGGT
TGCTCAGGTAAGAGAGTGCGCCGCACAATTAACCAGAGCGGCGAAAGCAAGTGGTATTTGTGTATTGATGGTGGGCCATG
TCACTAAAGATGGGCACTTAGCAGGTCCGCGTGTGTTAGAGCATATTGTTGATACCGTGCTCTACTTTGAGGGAGATACT
CATTCCTCATTCCGTTTAATACGCTCTATTAAAAATCGCTTTGGTGCAGTCAATGAGCTAGGTGTATTTGCGATGACGGA
AAAGGGTTTGCGAGGTGTGACTAACCCATCGGCAATTTTTTTGTCACAACATGAGCAGATGGTTCCTGGCGCATGTGTAC
TAGTAACTCAAGAGGGCAGTAGACCTTTGTTGGTGGAAATTCAGGCGTTAGTTGATACTGCGCATATACCTAATCCACGT
CGCTTAGCTGTTGGCTTAGAGCAAGCCCGTTTAGCAATGTTATTAGCGGTGCTGCACCGTCATGCAGGAGTGGCATGTTT
TGATCAAGATGTTTTCTTGAACGCGGTTGGTGGCGTAAAGATTTCTGAGCCAGCAGCTGATTTGGCGGTTTTGCTGGCAA
TTCAGTCATCTATTCGTAATCGTGCATTACCGAAGGAGTTGATTGTCTTTGGTGAGGTAGGCTTGGCGGGAGAAATTCGT
CCGTGCCCACGTGGTCAAGAGCGTCTTAAGGAGGCGGCAAAGCTTGGCTTTACTGTTGCCATTATTCCCAAGGCTAATAT
GCCGAAGGCAAAAATTCCTGGTTTGAAAGTCATTCCGGTCGAGCGAATTGATCAAGCAATTGCTGCTGCTGCCGAGTTAA
GCTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)

AAA domain (AAA_25)

(77-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.673

99.559

0.485
  radA Streptococcus pneumoniae Rx1

45.778

99.119

0.454
  radA Streptococcus pneumoniae D39

45.778

99.119

0.454
  radA Streptococcus pneumoniae R6

45.778

99.119

0.454
  radA Streptococcus pneumoniae TIGR4

45.778

99.119

0.454
  radA Streptococcus mitis SK321

45.778

99.119

0.454
  radA Streptococcus mitis NCTC 12261

45.556

99.119

0.452