Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NHB34_RS02940 Genome accession   NZ_CP099571
Coordinates   572069..573454 (-) Length   461 a.a.
NCBI ID   WP_353428119.1    Uniprot ID   -
Organism   Polynucleobacter sp. MWH-UH19D     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 567069..578454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHB34_RS02900 (NHB34_02895) - 567077..567409 (-) 333 WP_353428111.1 hypothetical protein -
  NHB34_RS02905 (NHB34_02900) - 567682..568746 (+) 1065 WP_353428112.1 helix-turn-helix domain-containing protein -
  NHB34_RS02910 (NHB34_02905) - 568782..569249 (+) 468 WP_353428113.1 polymer-forming cytoskeletal protein -
  NHB34_RS02915 (NHB34_02910) - 569266..569703 (+) 438 WP_353428114.1 hypothetical protein -
  NHB34_RS02920 (NHB34_02915) - 569719..569991 (-) 273 WP_353428115.1 hypothetical protein -
  NHB34_RS02925 (NHB34_02920) - 570264..570803 (+) 540 WP_353428116.1 hypothetical protein -
  NHB34_RS02930 (NHB34_02925) - 570803..571294 (+) 492 WP_353428117.1 hypothetical protein -
  NHB34_RS02935 (NHB34_02930) - 571316..571990 (+) 675 WP_353428118.1 diguanylate cyclase -
  NHB34_RS02940 (NHB34_02935) rpoN/rpoN1 572069..573454 (-) 1386 WP_353428119.1 RNA polymerase factor sigma-54 Machinery gene
  NHB34_RS02945 (NHB34_02940) - 573804..573998 (+) 195 WP_353428121.1 hypothetical protein -
  NHB34_RS02950 (NHB34_02945) - 574160..574582 (+) 423 WP_353428122.1 surface-adhesin E family protein -
  NHB34_RS02955 (NHB34_02950) - 574592..575350 (+) 759 WP_353428123.1 hypothetical protein -
  NHB34_RS02960 (NHB34_02955) - 575404..576213 (+) 810 WP_353428124.1 hypothetical protein -
  NHB34_RS02965 (NHB34_02960) - 576221..577249 (+) 1029 WP_353428125.1 hypothetical protein -
  NHB34_RS02970 (NHB34_02965) - 577246..577653 (+) 408 WP_353428126.1 hypothetical protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52161.40 Da        Isoelectric Point: 5.1245

>NTDB_id=700720 NHB34_RS02940 WP_353428119.1 572069..573454(-) (rpoN/rpoN1) [Polynucleobacter sp. MWH-UH19D]
MAISLNTRVSQTVSLTPQLQQSIKLLQLSNSELEQELAKAAEENPLLEFEPNPEIESNLSQSNERVEVIQQSWGPSQASQ
DDDEDWGERYERVAHKQTLLEYLDEQIHLLNINPEEQSLIAYMAGCLDDRGYLPEDLEAIHTDLASQLAEKTSLQHIEKA
LAKLQTLDPPGIGARDLAECLSLQIDRILETAKVDCAPWELAKEIVTTHLSKVGSKDWQKIKQASGKSEAEVLKAVELIR
TLQHNPGSQFERENDQWILPDVVVKLKNKRWVVESNPNAKPRLTLNNEYARILKENGQKKIDGALKQKMLEASWLVKNIA
QREETIQRVAQAIVERQQQFFSMGAIGMKPLVLREIAEELEMHESTISRVTTQKYLACPLGIFEFKYFFSSQVSTEKGGG
ISSTAIQALIKKIVEEESASKPISDTQIAQLLSEQGYIIARRTVAKYRDILRIPPVHLRKQ

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=700720 NHB34_RS02940 WP_353428119.1 572069..573454(-) (rpoN/rpoN1) [Polynucleobacter sp. MWH-UH19D]
ATGGCTATCTCACTCAATACCCGAGTCAGCCAGACGGTTAGCCTTACCCCGCAATTACAGCAATCCATCAAGCTTTTGCA
GCTCTCTAACTCTGAGCTGGAACAGGAACTGGCTAAAGCGGCTGAAGAAAACCCACTTCTAGAATTTGAGCCTAACCCCG
AAATTGAATCCAACCTCTCTCAATCGAATGAGCGGGTTGAGGTGATTCAACAGTCTTGGGGGCCTAGCCAAGCTTCCCAG
GATGACGATGAAGATTGGGGCGAGCGCTATGAGCGGGTTGCGCATAAGCAAACCCTACTAGAGTATTTGGATGAACAAAT
TCATCTTCTCAATATCAATCCTGAAGAGCAATCCTTAATTGCGTACATGGCTGGTTGTCTTGATGATCGCGGCTACCTAC
CTGAAGACCTTGAGGCCATTCATACCGATCTTGCAAGCCAATTGGCTGAAAAAACCTCGCTACAGCACATTGAAAAAGCG
CTAGCCAAACTGCAAACACTCGATCCACCCGGAATCGGTGCACGCGATCTTGCAGAATGCCTTTCTCTGCAAATTGATCG
CATTCTAGAAACTGCCAAAGTCGATTGCGCCCCATGGGAGTTGGCAAAAGAAATTGTGACGACCCATTTATCAAAGGTGG
GCTCTAAGGACTGGCAAAAAATCAAGCAGGCGAGCGGTAAATCAGAAGCAGAGGTACTCAAAGCCGTCGAACTCATTCGA
ACATTGCAACACAACCCAGGCTCTCAATTTGAGCGTGAGAACGATCAATGGATTTTGCCCGACGTTGTGGTCAAACTGAA
AAACAAGCGTTGGGTTGTGGAATCAAATCCGAATGCAAAACCTCGCCTCACTTTAAATAACGAGTACGCCAGAATTTTGA
AAGAGAATGGTCAAAAGAAAATTGATGGCGCTCTCAAACAAAAGATGCTCGAAGCCAGTTGGTTAGTAAAAAATATTGCG
CAACGCGAAGAAACAATTCAAAGAGTGGCGCAAGCGATTGTAGAGAGACAGCAGCAATTTTTCTCAATGGGTGCAATCGG
TATGAAACCACTTGTTCTACGTGAAATTGCCGAAGAGTTAGAAATGCATGAATCGACTATTTCACGGGTGACCACCCAAA
AGTATTTGGCCTGCCCTTTAGGAATTTTTGAATTCAAATACTTTTTCAGCAGTCAAGTGAGCACAGAAAAAGGCGGTGGC
ATTTCATCGACAGCAATACAGGCATTAATTAAAAAAATTGTCGAGGAAGAATCTGCCAGCAAGCCCATCTCAGATACCCA
GATTGCGCAACTATTAAGTGAGCAAGGCTATATCATTGCGCGCCGAACGGTAGCTAAGTACCGAGATATCTTGCGCATTC
CACCAGTTCATCTGCGCAAACAGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(92-289)

Sigma-54, DNA binding domain (Sigma54_DBD)

(305-460)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.158

100

0.484