Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NGM19_RS02890 Genome accession   NZ_CP099548
Coordinates   615173..615973 (+) Length   266 a.a.
NCBI ID   WP_151690577.1    Uniprot ID   -
Organism   Acinetobacter junii strain AJ6079     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 610173..620973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGM19_RS02865 (NGM19_02865) - 611083..612399 (+) 1317 WP_171493065.1 glycosyltransferase family 1 protein -
  NGM19_RS02870 (NGM19_02870) - 612427..612978 (+) 552 WP_151690576.1 phosphatase PAP2 family protein -
  NGM19_RS02875 (NGM19_02875) iscX 613061..613261 (+) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  NGM19_RS02880 (NGM19_02880) ndk 613368..613799 (+) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  NGM19_RS02885 (NGM19_02885) rlmN 613921..615156 (+) 1236 WP_042891255.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  NGM19_RS02890 (NGM19_02890) pilF 615173..615973 (+) 801 WP_151690577.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NGM19_RS02895 (NGM19_02895) - 615964..616755 (+) 792 WP_151690578.1 RodZ domain-containing protein -
  NGM19_RS02900 (NGM19_02900) ispG 616780..617895 (+) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NGM19_RS02905 (NGM19_02905) hisS 617892..619184 (+) 1293 WP_151690579.1 histidine--tRNA ligase -
  NGM19_RS02910 (NGM19_02910) - 619216..619914 (+) 699 WP_004950316.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30352.42 Da        Isoelectric Point: 9.5600

>NTDB_id=700604 NGM19_RS02890 WP_151690577.1 615173..615973(+) (pilF) [Acinetobacter junii strain AJ6079]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMDKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=700604 NGM19_RS02890 WP_151690577.1 615173..615973(+) (pilF) [Acinetobacter junii strain AJ6079]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGTTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAACATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGACAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.652

100

0.789
  pilF Acinetobacter baylyi ADP1

67.669

100

0.677