Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NFJ01_RS20175 Genome accession   NZ_CP099511
Coordinates   4234962..4236395 (-) Length   477 a.a.
NCBI ID   WP_252686828.1    Uniprot ID   -
Organism   Lelliottia amnigena strain P13     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4229962..4241395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ01_RS20145 (NFJ01_20145) elbB 4231479..4232132 (+) 654 WP_059177322.1 isoprenoid biosynthesis glyoxalase ElbB -
  NFJ01_RS20150 (NFJ01_20150) mtgA 4232129..4232854 (+) 726 WP_059177323.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NFJ01_RS20155 (NFJ01_20155) npr 4232909..4233181 (-) 273 WP_015960623.1 PTS phosphocarrier protein NPr -
  NFJ01_RS20160 (NFJ01_20160) rapZ 4233178..4234032 (-) 855 WP_015960622.1 RNase adapter RapZ -
  NFJ01_RS20165 (NFJ01_20165) ptsN 4234078..4234569 (-) 492 WP_059177324.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NFJ01_RS20170 (NFJ01_20170) hpf 4234652..4234939 (-) 288 WP_015960620.1 ribosome hibernation promoting factor -
  NFJ01_RS20175 (NFJ01_20175) rpoN/rpoN1 4234962..4236395 (-) 1434 WP_252686828.1 RNA polymerase factor sigma-54 Machinery gene
  NFJ01_RS20180 (NFJ01_20180) lptB 4236443..4237168 (-) 726 WP_059177326.1 LPS export ABC transporter ATP-binding protein -
  NFJ01_RS20185 (NFJ01_20185) lptA 4237175..4237729 (-) 555 WP_059177327.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NFJ01_RS20190 (NFJ01_20190) lptC 4237698..4238273 (-) 576 WP_131486412.1 LPS export ABC transporter periplasmic protein LptC -
  NFJ01_RS20195 (NFJ01_20195) kdsC 4238270..4238836 (-) 567 WP_059177329.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NFJ01_RS20200 (NFJ01_20200) kdsD 4238851..4239837 (-) 987 WP_059177330.1 arabinose-5-phosphate isomerase KdsD -
  NFJ01_RS20205 (NFJ01_20205) - 4239850..4240827 (-) 978 WP_059177331.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54036.86 Da        Isoelectric Point: 4.3643

>NTDB_id=700324 NFJ01_RS20175 WP_252686828.1 4234962..4236395(-) (rpoN/rpoN1) [Lelliottia amnigena strain P13]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQEKQDTETLDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFTETDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDSEIELDEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETQRLDEARLIISEHLDLLANHDFRSLMRVTR
LKEDVLKEAVSLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQGFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=700324 NFJ01_RS20175 WP_252686828.1 4234962..4236395(-) (rpoN/rpoN1) [Lelliottia amnigena strain P13]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAGTTGGCGATGACGCCACAGTTACAACAGGCAATCCGTCTGTTGCA
ACTGTCAACGTTAGAACTTCAGCAGGAACTGCAGCAAGCGCTGGACAGTAATCCACTGCTGGAGCAAACCGATCTTCATG
ATGAGGTAGATACCCAGGAAAAGCAGGACACCGAAACGCTCGATACGGCAGATGCCCTTGAGCAAAAAGAGATGCCTGAC
GAGTTACCGCTGGATGCCAGCTGGGATGAAATTTACACCGCTGGAACCCCATCCGGCACGCGCGCTGATTATCAGGATGA
TGAGCTACCGGTTTATCAGGGTGAAACTACGCAGTCGCTACAAGATTATCTGATGTGGCAGGTGGAATTGACCCCTTTCA
CCGAAACCGATCGCGCGATTGCCACGTCCATTGTCGATGCCGTGGATGACACCGGGTATTTAACGGTGACCCTGGACGAG
ATTCTGGAAAGCATGGGTGATAGCGAAATCGAACTCGACGAGATCGAAGCCGTTCTCAAGCGCATTCAACGCTTTGACCC
TGTCGGCGTCGCAGCAAAAGATCTGCGCGACTGCTTGCTGATCCAGCTTTCTCAGTTTGCAAAAGAGACGCAAAGACTTG
ACGAAGCGCGACTGATTATCAGCGAACATTTGGATTTGCTGGCGAATCATGATTTCCGCAGTCTGATGCGCGTTACGCGA
CTCAAAGAAGACGTGCTGAAAGAAGCGGTGAGTCTGATCCAGTCGCTCGATCCCCGTCCGGGCCAATCGATTCAAACCGG
CGAACCCGAATATGTGATTCCTGACGTCCTGGTCCGAAAGCACAACGGTCTCTGGACCGTAGAACTCAATTCCGACAGCA
TTCCCCGCCTGCAAATCAACCAGCAATACGCGTCTATGTGCACCAGTGCGCGGAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGCCTCGAAAGCCGAAATGATACGCTGCTACGCGTGAGTCGATGCAT
TGTGGAACAGCAGCAAGGCTTTTTTGAGCAGGGCGAAGAATTTATGAAACCGATGGTCCTGGCAGATATTGCTCAGGCCG
TTGAGATGCACGAATCGACCATTTCGCGTGTGACCACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGTCATGTGAATACCGAAGGCGGTGGCGAAGCCTCGTCGACGGCCATTCGTGCTCTGGTGAAGAAGTT
GATCGCCGCGGAAAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCCTGCTGTCCGATCAGGGTATCATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.49

100

0.486