Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PCP26_RS27385 Genome accession   NZ_CP115260
Coordinates   5748757..5750250 (+) Length   497 a.a.
NCBI ID   WP_003134760.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F026     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5743757..5755250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP26_RS27355 (PCP26_27340) - 5743969..5744778 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  PCP26_RS27360 (PCP26_27345) kdsD 5745192..5746172 (+) 981 WP_003134758.1 arabinose-5-phosphate isomerase KdsD -
  PCP26_RS27365 (PCP26_27350) - 5746172..5746711 (+) 540 WP_003134759.1 KdsC family phosphatase -
  PCP26_RS27370 (PCP26_27355) lptC 5746720..5747292 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  PCP26_RS27375 (PCP26_27360) lptA 5747279..5747806 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  PCP26_RS27380 (PCP26_27365) lptB 5747806..5748531 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  PCP26_RS27385 (PCP26_27370) rpoN/rpoN1 5748757..5750250 (+) 1494 WP_003134760.1 RNA polymerase factor sigma-54 Machinery gene
  PCP26_RS27390 (PCP26_27375) hpf 5750328..5750636 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PCP26_RS27395 (PCP26_27380) ptsN 5750650..5751114 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PCP26_RS27400 (PCP26_27385) rapZ 5751116..5751976 (+) 861 WP_003094361.1 RNase adapter RapZ -
  PCP26_RS27405 (PCP26_27390) - 5752006..5752278 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  PCP26_RS27410 (PCP26_27395) - 5752375..5753307 (-) 933 WP_003120918.1 ZIP family metal transporter -
  PCP26_RS27415 (PCP26_27400) - 5753326..5753937 (-) 612 WP_003098862.1 superoxide dismutase -
  PCP26_RS27420 (PCP26_27405) - 5753950..5754399 (-) 450 WP_017002538.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56064.25 Da        Isoelectric Point: 4.4327

>NTDB_id=699963 PCP26_RS27385 WP_003134760.1 5748757..5750250(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain F026]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQEPPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=699963 PCP26_RS27385 WP_003134760.1 5748757..5750250(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain F026]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCCCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGCCCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477