Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   NG745_RS08285 Genome accession   NZ_CP099465
Coordinates   1618811..1619710 (+) Length   299 a.a.
NCBI ID   WP_032871465.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ag75     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1613811..1624710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG745_RS08265 (NG745_08265) - 1614501..1616204 (+) 1704 WP_052827416.1 hypothetical protein -
  NG745_RS08270 (NG745_08270) - 1616201..1616482 (+) 282 WP_015239847.1 FlhB-like flagellar biosynthesis protein -
  NG745_RS08275 (NG745_08275) sucC 1616657..1617814 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  NG745_RS08280 (NG745_08280) sucD 1617843..1618745 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  NG745_RS08285 (NG745_08285) dprA 1618811..1619710 (+) 900 WP_032871465.1 DNA-processing protein DprA Machinery gene
  NG745_RS08290 (NG745_08290) topA 1619892..1621967 (+) 2076 WP_007409771.1 type I DNA topoisomerase -
  NG745_RS08295 (NG745_08295) trmFO 1622032..1623339 (+) 1308 WP_012117550.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  NG745_RS08300 (NG745_08300) xerC 1623409..1624326 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32865.01 Da        Isoelectric Point: 7.8285

>NTDB_id=699867 NG745_RS08285 WP_032871465.1 1618811..1619710(+) (dprA) [Bacillus velezensis strain Ag75]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHPLTVLSEGKTAPEAIFREIERKDPELDEVLSDYRREGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTLLLEKGRKIGIVGTRKPTEDGIKAVGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=699867 NG745_RS08285 WP_032871465.1 1618811..1619710(+) (dprA) [Bacillus velezensis strain Ag75]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCATTCTCTGTCTTTTTTACCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGGATCCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCGAAGGCATTACTGTCATT
CCGATTTCATCAAGCCGCTATCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGGAACAC
GCTGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGTTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGATTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGATTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCTCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.903

100

0.709
  dprA Lactococcus lactis subsp. cremoris KW2

41.667

88.294

0.368