Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NF199_RS12190 Genome accession   NZ_CP099460
Coordinates   2518185..2518799 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain E     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2513185..2523799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF199_RS12160 rpsT 2513490..2513756 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  NF199_RS12165 holA 2513773..2514814 (-) 1042 Protein_2348 DNA polymerase III subunit delta -
  NF199_RS12170 - 2514854..2515005 (-) 152 Protein_2349 hypothetical protein -
  NF199_RS12175 - 2515046..2515180 (+) 135 WP_003152870.1 YqzM family protein -
  NF199_RS12180 - 2515199..2517548 (-) 2350 Protein_2351 DNA internalization-related competence protein ComEC/Rec2 -
  NF199_RS12185 - 2517549..2518118 (-) 570 WP_003152868.1 ComE operon protein 2 -
  NF199_RS12190 comEA 2518185..2518799 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  NF199_RS12195 comER 2518858..2519679 (+) 822 WP_012118027.1 late competence protein ComER -
  NF199_RS12200 - 2519748..2520485 (-) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  NF199_RS12205 rsfS 2520482..2520838 (-) 357 WP_007408260.1 ribosome silencing factor -
  NF199_RS12210 yqeK 2520842..2521417 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NF199_RS12215 - 2521407..2521976 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  NF199_RS12220 yhbY 2521987..2522277 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  NF199_RS12225 aroE 2522271..2523113 (-) 843 WP_265605838.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=699794 NF199_RS12190 WP_032873955.1 2518185..2518799(-) (comEA) [Bacillus velezensis strain E]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=699794 NF199_RS12190 WP_032873955.1 2518185..2518799(-) (comEA) [Bacillus velezensis strain E]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363