Detailed information NTG0699766

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: NF199_RS05390
• Genome accession: NZ_CP099460
• Coordinates: 1041623..1044652 (+)
• Length: 1009 a.a.
• NCBI ID: WP_409365244.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain E
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1036623..1049652

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NF199_RS05375	-	1036704..1037213 (-)	510	WP_014304752.1	ferritin-like domain-containing protein	-
NF199_RS05380	-	1037282..1037995 (-)	714	WP_079891127.1	DUF421 domain-containing protein	-
NF199_RS05385	addB	1038136..1041636 (+)	3501	WP_032874392.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
NF199_RS05390	addA	1041623..1044652 (+)	3030	WP_409365244.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
NF199_RS19680	-	1044550..1045329 (+)	780	Protein_999	PD-(D/E)XK nuclease family protein	-
NF199_RS05400	sbcD	1045394..1046566 (+)	1173	WP_032874394.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1009 a.a.
• Molecular weight: 115645.80 Da
• Isoelectric Point: 6.3278

>NTDB_id=699766 NF199_RS05390 WP_409365244.1 1041623..1044652(+) (addA) [Bacillus velezensis strain E]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAATGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELADRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLQSFVRLYDVTEESKMEELAFYQYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNFLDDLQQIEELISCRHDFDALYERVPAVSFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKVK
TDYFTRSPQDHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAVDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEETGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNEAAKTELLLIDSADGADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANADRHDELYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRTPGLSSPEDIARDPSRFHIRIFADKV
NCWKRIQKNVLKKRANVSKRFSRASRFLIRFHLMIRRAVCLNGNIRTES

Nucleotide

• Length: 3030 bp

>NTDB_id=699766 NF199_RS05390 WP_409365244.1 1041623..1044652(+) (addA) [Bacillus velezensis strain E]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCCGCCGCGACCGGATCAGGTAAAACGGCCGTGCTTGTGGAACGGATGATCAGAAAGATAACTGCGGAAGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAGATGAAGCATCGGATCGCGGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTGCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAAGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCCGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAAGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGCGGACAGATATACGACAGACAGGCATGATCTGGATCTTCAAGACCTTGTCAAACGGGTGTACGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGCAAAGCTTTGTCCGCCTGTATGACGTGACGGAAGAAAGCAAAATGG
AAGAGCTTGCGTTTTACCAATATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTTGAAAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCCCGCGCGCTGATAATTTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTCGATGCGCTGTATGAACGGGTTCCCGCAGTCTCATTTAAGCGGGCGAAGGCGGTAAAGG
GTGATGAATTTGACAAAGCGCTTTTGGACGAAGCGACCGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGGTAAAG
ACAGATTACTTTACAAGAAGTCCGCAAGATCACTTAAAGAGTTTAGCAGATATGAAGCCTGTCATCGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATTATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGGTTGATGAAGAAGGCCGGCGGGTGCCGAGCGAAGCGGCCGTTTATTATCAAGACCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACGAACCTTGTGCAGGAGTCTATCCTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGACGGGAAATCTGTTTATGGTAGGCGACGTCAAACAATCGATCTACCGTTTCAGACTTGCTGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAGAGCGGGGAAGGCGCGGGGCAGAAAATTGATCTGAACCAAAATTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGAAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAGCTCGGCGCATCGTATCCGCCGAATGAAGCGGCAAAAACGGAACTGCTGCTGATTGACAGCGCTG
ATGGCGCAGACAGCTCCGAGGATGCCGAAGACTTTGAAACCGTGCACTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGA
AAGCTTGTCTCATCTCCGTTTAAGGTCTATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTAAT
TTTGCTGCGCTCCATGCCGTGGGCGCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTTTACGCCAATTTGA
CGTCGGGTTACTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTGAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGCTCGCCCATCGTCGGATGTGACGAAAATGAACTTGCGCTGATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGCTGACAGGCATGATGAACTGTATCAAAAACTGAGAACCT
TTTATGACAGTCTGCAAAAGTGGCGGTCATTTTCGACGAATCATTCGGTGTCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGATATTTTGACTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACGGCCCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTTCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTACATTCATCCGGAACTCAGAATCAGCTATCCGACTCTTCCGCTTGTGGCGATGAAGAAAA
AGATGCGCAGAGAGCTCTTATCCGAAGAATTGCGTGTATTGTATGTGGCGCTGACCAGAGCGAAGGAAAAGCTGTTTCTC
GTCGGCTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCTGATTGGCTTCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGGCCGGCGCTCATCCGTCACCGTGACATGGAAGCGCACA
GGACGCCGGGGCTTTCATCGCCTGAGGATATCGCGCGCGATCCGTCGCGTTTTCACATCCGGATCTTTGCAGACAAAGTG
AATTGCTGGAAGAGAATCCAAAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAAGCGATTCAGCAGGGCGAGCCGATT
CCTGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTGCTTGAATGGGAATATCCGTACCGAGAGCTGA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-881)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	84.519	94.747	0.801