Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NG694_RS10165 Genome accession   NZ_CP099457
Coordinates   2016429..2017802 (+) Length   457 a.a.
NCBI ID   WP_003723898.1    Uniprot ID   L8DQ47
Organism   Listeria monocytogenes strain L1812     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2011429..2022802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG694_RS10145 (NG694_10140) - 2011784..2012242 (+) 459 WP_003723894.1 CtsR family transcriptional regulator -
  NG694_RS10150 (NG694_10145) - 2012255..2012773 (+) 519 WP_003732822.1 UvrB/UvrC motif-containing protein -
  NG694_RS10155 (NG694_10150) - 2012770..2013792 (+) 1023 WP_010989379.1 protein arginine kinase -
  NG694_RS10160 (NG694_10155) - 2013821..2016283 (+) 2463 WP_003723897.1 ATP-dependent Clp protease ATP-binding subunit -
  NG694_RS10165 (NG694_10160) radA 2016429..2017802 (+) 1374 WP_003723898.1 DNA repair protein RadA Machinery gene
  NG694_RS10170 (NG694_10165) - 2017936..2019009 (+) 1074 WP_003723899.1 PIN/TRAM domain-containing protein -
  NG694_RS10175 (NG694_10170) ispD 2019029..2019727 (+) 699 WP_010989381.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NG694_RS10180 (NG694_10175) ispF 2019720..2020193 (+) 474 WP_009930888.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  NG694_RS10185 (NG694_10180) gltX 2020212..2021687 (+) 1476 WP_003732826.1 glutamate--tRNA ligase -
  NG694_RS10190 (NG694_10185) epsC 2022085..2022699 (+) 615 WP_003726845.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49991.63 Da        Isoelectric Point: 7.9613

>NTDB_id=699742 NG694_RS10165 WP_003723898.1 2016429..2017802(+) (radA) [Listeria monocytogenes strain L1812]
MAKAKRTTKFVCQACGYESAKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASETEKRVETNMPELN
RVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQE
TIDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTATLMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFG
NAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTG
EIRRVARIEQRVQEAAKLGFKRIFIPKNNEGNWKIPKDVQVVGVETIGEALKKALPD

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=699742 NG694_RS10165 WP_003723898.1 2016429..2017802(+) (radA) [Listeria monocytogenes strain L1812]
ATGGCTAAAGCAAAAAGGACAACCAAATTTGTGTGTCAGGCATGTGGATATGAATCGGCAAAATGGATGGGAAAATGTCC
GAATTGCAACGAGTGGAATCAGATGGTAGAAGCCTTAGAACCGTCAAAAAAATCACGCTCAGCTTTCAATCATACAGGAG
AGCCTTCAAAAGCGACTCCAATTACTCAAATAGCAAGTGAAACAGAAAAACGAGTTGAAACAAATATGCCCGAGTTAAAT
AGAGTTCTTGGTGGCGGTGTGGTTCCTGGGTCTATGGTACTTGTCGGAGGAGACCCTGGTATTGGTAAATCAACATTGTT
ATTACAAGTATCAGCGCAACTCACACTTACAAATAAAAAAGTATTGTATATCTCAGGAGAAGAATCAATCAAACAAACGA
AGCTACGGGCAGAACGCTTGCAAGTTTCGGGAGATAATTTATATGTGTATGCAGAAACAAATTTAGAAGCAGTTCAAGAA
ACGATTGATTTTGTAAAACCTGATTTTGTAGTCATTGACTCTATTCAGACTGTTTATCATCCTGATGTTACAAGTGCCGC
AGGGAGTGTTTCTCAAGTTAGGGAATGTACAGCGACATTGATGCGAATTGCTAAAATGCAAAACATCGCTATCTTTATTG
TTGGGCATGTAACTAAGGAAGGTGCTATTGCGGGACCGCGCCTATTAGAACATATGGTTGATACTGTACTCTATTTTGAA
GGGGAGCGTCACCATGCTTACCGGATTTTGCGTGCAGTGAAAAACCGTTTTGGTTCCACTAACGAAATGGGTATATTTGA
AATGCGAGATGTAGGACTTGTTGAAGTTGCTAATCCTTCCGAGGTATTCTTGGAAGAACGTCTTGAAGGCGCTTCAGGCT
CAACGGTTGTAGCTTCTATGGAAGGAACTCGCCCCGTCCTTGTGGAAATACAAGCGCTTGTTTCGCCAACGATGTTCGGT
AATGCTAAGCGAATGGCGACGGGAATAGACTACAACAAAGTTTCGCTAATTATGGCTGTTTTAGAAAAGCGAGTAGGTTT
AATGTTGCAAAACCAAGATGCTTATTTGAAAGCGGCGGGCGGGGTTAAATTGGATGAGCCTGCAGTGGATTTGGCGGTTG
CAGTCAGTGTTGCATCTAGTTATCGTGATAAACCAACAAGAAGTACGGACTGCTTTATTGGGGAACTTGGACTTACAGGT
GAAATTCGTCGTGTAGCAAGAATTGAACAACGCGTACAAGAAGCAGCAAAACTTGGCTTTAAGCGAATTTTTATTCCTAA
AAATAACGAAGGTAATTGGAAAATACCGAAAGACGTGCAAGTGGTTGGGGTGGAAACGATTGGAGAAGCTTTGAAGAAAG
CTTTACCAGATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L8DQ47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.421

99.781

0.683
  radA Streptococcus pneumoniae Rx1

65.265

98.906

0.646
  radA Streptococcus pneumoniae D39

65.265

98.906

0.646
  radA Streptococcus pneumoniae R6

65.265

98.906

0.646
  radA Streptococcus pneumoniae TIGR4

65.265

98.906

0.646
  radA Streptococcus mitis NCTC 12261

65.265

98.906

0.646
  radA Streptococcus mitis SK321

65.044

98.906

0.643