Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   NE411_RS20250 Genome accession   NZ_CP099450
Coordinates   3854758..3855885 (+) Length   375 a.a.
NCBI ID   WP_000470750.1    Uniprot ID   A0A2C3GXQ0
Organism   Bacillus pacificus strain ANSB901     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3849758..3860885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NE411_RS20220 (NE411_20175) spoIIIJ 3849804..3850571 (-) 768 WP_000726761.1 YidC family membrane integrase SpoIIIJ -
  NE411_RS20225 (NE411_20180) rnpA 3850624..3850971 (-) 348 WP_000726627.1 ribonuclease P protein component -
  NE411_RS20230 (NE411_20185) rpmH 3851048..3851182 (-) 135 WP_000831901.1 50S ribosomal protein L34 -
  NE411_RS20235 (NE411_20190) dnaA 3851748..3853088 (+) 1341 WP_000428025.1 chromosomal replication initiator protein DnaA -
  NE411_RS20240 (NE411_20195) dnaN 3853267..3854406 (+) 1140 WP_001212895.1 DNA polymerase III subunit beta -
  NE411_RS20245 (NE411_20200) yaaA 3854534..3854746 (+) 213 WP_000821367.1 S4 domain-containing protein YaaA -
  NE411_RS20250 (NE411_20205) recF 3854758..3855885 (+) 1128 WP_000470750.1 DNA replication/repair protein RecF Machinery gene
  NE411_RS20255 (NE411_20210) gyrB 3855924..3857846 (+) 1923 WP_000435991.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  NE411_RS20260 (NE411_20215) gyrA 3857935..3860406 (+) 2472 WP_001282851.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 43334.66 Da        Isoelectric Point: 6.7809

>NTDB_id=699605 NE411_RS20250 WP_000470750.1 3854758..3855885(+) (recF) [Bacillus pacificus strain ANSB901]
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGTKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRA

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=699605 NE411_RS20250 WP_000470750.1 3854758..3855885(+) (recF) [Bacillus pacificus strain ANSB901]
TTGTTTATTTCAGAAATACAATTAAAAAACTATCGCAATTATGAAAAGTTAGAGCTTTCCTTTGAAGATAAGGTAAATGT
AATTATCGGCGAGAATGCGCAAGGGAAAACGAATTTGATGGAAGCTATTTATGTTTTAGCGATGGCGAAATCTCATAGAA
CCTCTAATGATCGCGAGCTTATCCGTTGGGATGAAGATTTCGGTCAAATTAAAGGAAAGCTACAAAAGAGAAATAGTTCT
TTGTCTTTGGAATTAAATATCTCGAAAAAAGGTAAAAAGGCAAAATTAAATCAACTTGAACAACAAAAATTGAGTCAATA
TATTGGCGTGATGAATGTTGTCATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGACGCT
TTTTAGATATGGAATTAGGCCAAATAGCTCCGGTCTATTTGTATGAACTGAGTCAATATCAAAAGGTGCTCACACAACGA
AATCACTTGCTAAAAAAGATGCAAGGGAATAGTAAGAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTTATTGA
GCATGGTACAAAAATTTTGCAAAAACGCTTTGAATTTTTGCATTTACTACAAGAATGGGCAGCTCCAATTCATCGTGGTA
TAAGCCGTGGGTTAGAAGAATTAGAAATTGTCTATAAACCGAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCCGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTAATTGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCGCAAGGACAACAACGAACGACCGCACTGT
CCCTAAAATTAGCTGAAATTGAATTGATTTACTCAGAAGTTAAAGAATATCCGATTCTTCTATTAGATGATGTATTATCA
GAATTAGATGATTATCGTCAATCGCATCTGTTAAATACAATTCAAGGAAAAGTGCAAACATTTGTGACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTAAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGATA
GGGCGTAA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2C3GXQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

66.304

98.133

0.651