Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NE411_RS14110 Genome accession   NZ_CP099450
Coordinates   2676056..2677099 (-) Length   347 a.a.
NCBI ID   WP_001013195.1    Uniprot ID   A0AAP5FWA8
Organism   Bacillus pacificus strain ANSB901     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2671056..2682099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NE411_RS14075 (NE411_14055) - 2671941..2672288 (+) 348 WP_000859168.1 helix-turn-helix transcriptional regulator -
  NE411_RS14080 (NE411_14060) - 2672493..2673662 (+) 1170 WP_000190219.1 site-specific integrase -
  NE411_RS14085 - 2673816..2674004 (-) 189 Protein_2815 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NE411_RS14090 (NE411_14065) comGE 2673974..2674276 (-) 303 WP_000829518.1 competence type IV pilus minor pilin ComGE -
  NE411_RS14095 (NE411_14070) comGD 2674269..2674724 (-) 456 WP_000810388.1 comG operon protein ComGD -
  NE411_RS14100 (NE411_14075) comGC 2674721..2675020 (-) 300 WP_001178694.1 competence type IV pilus major pilin ComGC -
  NE411_RS14105 (NE411_14080) comGB 2675032..2676063 (-) 1032 WP_000473549.1 competence type IV pilus assembly protein ComGB -
  NE411_RS14110 (NE411_14085) comGA 2676056..2677099 (-) 1044 WP_001013195.1 competence protein ComGA Machinery gene
  NE411_RS14115 (NE411_14090) - 2677305..2678000 (+) 696 WP_000434106.1 helix-turn-helix transcriptional regulator -
  NE411_RS14120 (NE411_14095) - 2678126..2678368 (+) 243 WP_000440713.1 DUF2626 domain-containing protein -
  NE411_RS14125 (NE411_14100) - 2678476..2679870 (+) 1395 WP_001094339.1 L-cystine transporter -
  NE411_RS14130 (NE411_14105) - 2680269..2680484 (-) 216 WP_001008330.1 DUF3912 family protein -
  NE411_RS14135 (NE411_14110) - 2680738..2681049 (+) 312 WP_001093243.1 hypothetical protein -
  NE411_RS14140 (NE411_14115) - 2681086..2681574 (-) 489 WP_000764259.1 hypothetical protein -
  NE411_RS14145 (NE411_14120) - 2681654..2682028 (-) 375 WP_001012337.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39359.83 Da        Isoelectric Point: 9.0210

>NTDB_id=699589 NE411_RS14110 WP_001013195.1 2676056..2677099(-) (comGA) [Bacillus pacificus strain ANSB901]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKQYIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
IQIDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTVCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESSLRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=699589 NE411_RS14110 WP_001013195.1 2676056..2677099(-) (comGA) [Bacillus pacificus strain ANSB901]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTAAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGTCAGAAGGATGTAGTGGTTCAACTGCGCATAGGAAAGGATTTAATGACGAAACAATACATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGAGAGAGGCGGAAACCACAAAACGGTTCACTATAT
ATACAAATAGATGGACAAGAAGTGTATTTACGCCTTTCCACGCTTCCAACCGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCGTCTATTCAGCCGCTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
ATTATTCCCATGGATTACTCGTATTTACGGGACCGACTGGTTCAGGAAAGACAACAACAATGTATGCATTACTAGAGGTG
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGATGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTGG
GTGAAATTCGTGATGAAGAAACAGCAAAAATCGCTATAAGAGCAAGTTTGACTGGTCATTTAGTAATGACGACACTGCAT
ACGAATGATGCGAAAGGAGCAATACTTCGGTTCATGGATTTTGGTATAACGAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTTGAATTGAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTGTATGTAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACGTACAAGCATGAGACATTAGAGTCTTCGCTGCGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366