Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NE411_RS12515 Genome accession   NZ_CP099450
Coordinates   2417605..2418039 (-) Length   144 a.a.
NCBI ID   WP_000811499.1    Uniprot ID   A0A1J9ZQC5
Organism   Bacillus pacificus strain ANSB901     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2412605..2423039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NE411_RS12500 (NE411_12485) - 2413629..2414186 (+) 558 WP_000062077.1 PadR family transcriptional regulator -
  NE411_RS12505 (NE411_12490) metE 2414854..2417142 (+) 2289 WP_001007594.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  NE411_RS12510 (NE411_12495) comJ 2417180..2417569 (-) 390 WP_000424014.1 competence protein ComJ -
  NE411_RS12515 (NE411_12500) nucA/comI 2417605..2418039 (-) 435 WP_000811499.1 DNA-entry nuclease Machinery gene
  NE411_RS12520 (NE411_12505) - 2418429..2419178 (+) 750 WP_000388461.1 DUF3967 domain-containing protein -
  NE411_RS12525 (NE411_12510) kinB 2419232..2420506 (-) 1275 WP_000420388.1 sporulation sensor histidine kinase KinB -
  NE411_RS12530 (NE411_12515) - 2420816..2421016 (+) 201 WP_000929285.1 hypothetical protein -
  NE411_RS12535 (NE411_12520) - 2421140..2422303 (+) 1164 WP_000434558.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15996.35 Da        Isoelectric Point: 8.4331

>NTDB_id=699586 NE411_RS12515 WP_000811499.1 2417605..2418039(-) (nucA/comI) [Bacillus pacificus strain ANSB901]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKDRYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=699586 NE411_RS12515 WP_000811499.1 2417605..2418039(-) (nucA/comI) [Bacillus pacificus strain ANSB901]
ATGAAACAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTGCTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGATCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCGCATATTGAATATATAAGTCCAGCCGATAACCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J9ZQC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

72.072

77.083

0.556