Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   L1F06_RS03915 Genome accession   NZ_CP099397
Coordinates   860175..861536 (+) Length   453 a.a.
NCBI ID   WP_129482845.1    Uniprot ID   -
Organism   Pseudomonas hydrolytica strain DSWY01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 855175..866536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1F06_RS03885 (L1F06_003885) - 855540..856859 (+) 1320 WP_129482840.1 metallopeptidase TldD-related protein -
  L1F06_RS03890 (L1F06_003890) - 856865..857989 (-) 1125 WP_129482841.1 class I SAM-dependent methyltransferase -
  L1F06_RS03895 (L1F06_003895) - 858059..858835 (+) 777 WP_129482842.1 ferredoxin--NADP reductase -
  L1F06_RS03900 (L1F06_003900) - 858947..859189 (+) 243 WP_003242693.1 YdcH family protein -
  L1F06_RS03905 (L1F06_003905) - 859268..859546 (+) 279 WP_129482843.1 DUF465 domain-containing protein -
  L1F06_RS03910 (L1F06_003910) - 859586..860080 (-) 495 WP_129482844.1 GNAT family N-acetyltransferase -
  L1F06_RS03915 (L1F06_003915) radA 860175..861536 (+) 1362 WP_129482845.1 DNA repair protein RadA Machinery gene
  L1F06_RS03920 (L1F06_003920) - 861720..861872 (+) 153 WP_177491148.1 hypothetical protein -
  L1F06_RS03925 (L1F06_003925) - 861879..862250 (-) 372 WP_003242702.1 PilZ domain-containing protein -
  L1F06_RS03930 (L1F06_003930) - 862416..862922 (+) 507 WP_003242703.1 DUF3015 domain-containing protein -
  L1F06_RS03935 (L1F06_003935) glyA 863088..864341 (-) 1254 WP_003242705.1 serine hydroxymethyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48406.95 Da        Isoelectric Point: 7.0802

>NTDB_id=699471 L1F06_RS03915 WP_129482845.1 860175..861536(+) (radA) [Pseudomonas hydrolytica strain DSWY01]
MAKAKRMYGCTECGATFPKWAGQCGECGAWNTLVETVLEGASSPSGRTGWAGQQAALKTLAEVSVEEVPRFSTASGELDR
VLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIATRFPALYVTGEESQQQVAMRARRLGLPEDKLKVMTETCIESIIATA
RLEKPKVMVIDSIQTIFTEQLQSAPGGVAQVRESAALLVRFAKQSGTAVFLVGHVTKEGALAGPRVLEHMVDTVLYFEGE
SDGRLRLLRAVKNRFGAINELGVFGMTDKGLKEVTNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDTSHLANP
RRVTLGLDQNRLAMLLAVLHRHGGIPTYDQDVFLNVVGGVKVLETASDLALMAAVISSLRNRPLPHDLLVFGEVGLSGEI
RPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLSIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=699471 L1F06_RS03915 WP_129482845.1 860175..861536(+) (radA) [Pseudomonas hydrolytica strain DSWY01]
ATGGCCAAGGCAAAACGCATGTACGGCTGCACCGAGTGCGGCGCCACCTTTCCCAAATGGGCAGGGCAGTGTGGCGAGTG
CGGGGCCTGGAACACCCTGGTGGAAACCGTGCTGGAAGGCGCTTCGTCGCCGAGCGGGCGTACCGGCTGGGCTGGCCAGC
AGGCGGCGCTGAAGACCCTGGCCGAGGTCAGCGTCGAGGAAGTGCCGCGCTTTTCCACCGCCTCCGGCGAGCTGGACCGC
GTGCTCGGCGGCGGCTTGGTGGACGGCTCGGTGGTGCTGATCGGCGGCGACCCCGGCATCGGCAAGTCGACCATTCTGCT
GCAGACCCTGTGCAATATCGCCACGCGCTTTCCGGCGCTGTACGTCACCGGCGAGGAGTCGCAGCAGCAGGTGGCCATGC
GCGCCCGTCGTCTGGGCCTGCCCGAGGACAAGCTGAAGGTGATGACCGAGACCTGCATCGAGTCGATCATCGCCACCGCG
CGCCTGGAAAAGCCCAAGGTCATGGTGATCGACTCGATCCAGACCATCTTCACCGAGCAGCTGCAGTCGGCGCCCGGCGG
CGTGGCCCAAGTGCGCGAAAGCGCCGCCCTGCTGGTGCGCTTCGCCAAGCAGAGCGGTACCGCGGTCTTTCTGGTCGGCC
ACGTGACCAAGGAAGGCGCGCTGGCCGGCCCGCGCGTGCTGGAGCACATGGTCGACACCGTGCTGTATTTCGAGGGCGAA
TCCGACGGTCGCCTGCGCCTGCTGCGGGCAGTGAAAAACCGCTTCGGCGCGATCAACGAGCTGGGCGTGTTCGGCATGAC
CGACAAGGGCCTGAAAGAGGTTACCAACCCCTCGGCGATCTTCCTCACCCGCGCCCAGGAGGAGGTGCCGGGCAGCGTGG
TGATGGCCACCTGGGAAGGCACCCGGCCGATGCTGGTGGAGGTGCAGGCACTGGTCGACACCAGCCACCTGGCCAACCCG
CGGCGCGTCACCCTGGGCCTGGATCAGAACCGCCTGGCCATGCTGCTGGCCGTGCTGCATCGTCACGGTGGTATTCCCAC
CTACGATCAGGACGTGTTCCTCAACGTGGTCGGCGGGGTCAAGGTGCTGGAGACCGCCTCGGACCTGGCCCTGATGGCGG
CGGTGATCTCGAGTCTGCGCAACCGTCCGTTGCCGCACGATCTGCTGGTGTTTGGCGAGGTGGGGCTGTCCGGCGAGATC
CGCCCGGTACCGAGCGGTCAGGAGCGTCTGAAGGAGGCCGCCAAGCATGGCTTCAAGCGCGCCATCGTGCCCAAGGGCAA
TGCGCCCAAGGAGGCGCCGGCGGGGCTATCGATCATTGCCGTGACACGCCTGGAGCAGGCGCTGGATGCGTTGTTCGAGT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(361-427)

AAA domain (AAA_25)

(77-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.011

100

0.492
  radA Streptococcus pneumoniae Rx1

46.725

100

0.472
  radA Streptococcus pneumoniae D39

46.725

100

0.472
  radA Streptococcus pneumoniae TIGR4

46.725

100

0.472
  radA Streptococcus pneumoniae R6

46.725

100

0.472
  radA Streptococcus mitis NCTC 12261

46.507

100

0.47
  radA Streptococcus mitis SK321

46.696

100

0.468