Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FJI53_RS02190 Genome accession   NZ_AP023237
Coordinates   473312..473989 (-) Length   225 a.a.
NCBI ID   WP_001377644.1    Uniprot ID   -
Organism   Escherichia coli strain F070     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468312..478989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJI53_RS02175 chiA 469679..472372 (+) 2694 WP_001377643.1 bifunctional chitinase/lysozyme -
  FJI53_RS02180 bfd 472541..472735 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  FJI53_RS02185 bfr 472807..473283 (+) 477 WP_000675504.1 bacterioferritin -
  FJI53_RS02190 pilD 473312..473989 (-) 678 WP_001377644.1 prepilin peptidase GspO Machinery gene
  FJI53_RS02195 gspM 473989..474450 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  FJI53_RS02200 gspL 474447..475610 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  FJI53_RS02205 gspK 475625..476608 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  FJI53_RS02210 gspJ 476601..477188 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  FJI53_RS02215 gspI 477181..477558 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  FJI53_RS02220 gspH 477555..478064 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  FJI53_RS02225 gspG 478072..478509 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.75 Da        Isoelectric Point: 7.8986

>NTDB_id=69947 FJI53_RS02190 WP_001377644.1 473312..473989(-) (pilD) [Escherichia coli strain F070]
MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=69947 FJI53_RS02190 WP_001377644.1 473312..473989(-) (pilD) [Escherichia coli strain F070]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTGTTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382
  pilD Vibrio cholerae strain A1552

35.745

100

0.373