Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PCP14_RS04530 Genome accession   NZ_CP115251
Coordinates   947121..948614 (+) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain F035     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 942121..953614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP14_RS04500 (PCP14_04490) - 942334..943143 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  PCP14_RS04505 (PCP14_04495) kdsD 943556..944536 (+) 981 WP_003134758.1 arabinose-5-phosphate isomerase KdsD -
  PCP14_RS04510 (PCP14_04500) - 944536..945075 (+) 540 WP_003094350.1 KdsC family phosphatase -
  PCP14_RS04515 (PCP14_04505) lptC 945084..945656 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  PCP14_RS04520 (PCP14_04510) lptA 945643..946170 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  PCP14_RS04525 (PCP14_04515) lptB 946170..946895 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  PCP14_RS04530 (PCP14_04520) rpoN/rpoN1 947121..948614 (+) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  PCP14_RS04535 (PCP14_04525) hpf 948692..949000 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PCP14_RS04540 (PCP14_04530) ptsN 949014..949478 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PCP14_RS04545 (PCP14_04535) rapZ 949480..950340 (+) 861 WP_003094361.1 RNase adapter RapZ -
  PCP14_RS04550 (PCP14_04540) - 950370..950642 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  PCP14_RS04555 (PCP14_04545) - 950739..951671 (-) 933 WP_003120918.1 ZIP family metal transporter -
  PCP14_RS04560 (PCP14_04550) - 951690..952301 (-) 612 WP_009315925.1 superoxide dismutase -
  PCP14_RS04565 (PCP14_04555) - 952314..952763 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=698997 PCP14_RS04530 WP_003094357.1 947121..948614(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain F035]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=698997 PCP14_RS04530 WP_003094357.1 947121..948614(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain F035]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCCCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
TCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGATAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCTGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477