Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HXS64_RS03205 Genome accession   NZ_AP023235
Coordinates   699012..699689 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain YJ6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 694012..704689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXS64_RS03190 chiA 695378..698071 (+) 2694 WP_065898742.1 bifunctional chitinase/lysozyme -
  HXS64_RS03195 bfd 698240..698434 (+) 195 WP_047603876.1 bacterioferritin-associated ferredoxin -
  HXS64_RS03200 bfr 698507..698983 (+) 477 WP_000675504.1 bacterioferritin -
  HXS64_RS03205 pilD 699012..699689 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  HXS64_RS03210 gspM 699689..700150 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  HXS64_RS03215 gspL 700147..701310 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  HXS64_RS03220 gspK 701325..702308 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  HXS64_RS03225 gspJ 702301..702888 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  HXS64_RS03230 gspI 702881..703258 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  HXS64_RS03235 gspH 703255..703764 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  HXS64_RS03240 gspG 703772..704209 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=69877 HXS64_RS03205 WP_000178161.1 699012..699689(-) (pilD) [Escherichia coli strain YJ6]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=69877 HXS64_RS03205 WP_000178161.1 699012..699689(-) (pilD) [Escherichia coli strain YJ6]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment