Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EP162_RS03250 Genome accession   NZ_AP018524
Coordinates   768832..770649 (+) Length   605 a.a.
NCBI ID   WP_013773638.1    Uniprot ID   -
Organism   Melissococcus plutonius strain DAT585     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 763832..775649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP162_RS03230 (DAT585_0659) trpS 764781..765788 (-) 1008 WP_013773634.1 tryptophan--tRNA ligase -
  EP162_RS03235 (DAT585_0660) spxA 766113..766511 (+) 399 WP_015695165.1 transcriptional regulator SpxA -
  EP162_RS03240 (DAT585_0661) - 766756..767409 (+) 654 WP_013773636.1 adaptor protein MecA -
  EP162_RS03245 (DAT585_0662) - 767612..768790 (+) 1179 WP_013773637.1 competence protein CoiA -
  EP162_RS03250 (DAT585_0663) pepF 768832..770649 (+) 1818 WP_013773638.1 oligoendopeptidase F Regulator
  EP162_RS03255 (DAT585_0664) - 770749..771399 (-) 651 WP_048589730.1 DsbA family protein -
  EP162_RS03260 (DAT585_0665) - 771502..772095 (-) 594 WP_048589731.1 CYTH domain-containing protein -
  EP162_RS03265 (DAT585_0666) - 772276..772950 (+) 675 Protein_667 GTP pyrophosphokinase -
  EP162_RS03270 (DAT585_0667) - 772971..773768 (+) 798 WP_013773645.1 NAD kinase -
  EP162_RS03275 (DAT585_0668) - 773778..774674 (+) 897 WP_013773646.1 RluA family pseudouridine synthase -
  EP162_RS03280 (DAT585_0669) tsaE 774845..775330 (+) 486 WP_013773647.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -

Sequence

Protein


Download         Length: 605 a.a.        Molecular weight: 70364.61 Da        Isoelectric Point: 5.5181

>NTDB_id=69844 EP162_RS03250 WP_013773638.1 768832..770649(+) (pepF) [Melissococcus plutonius strain DAT585]
MEKIKQLPNRSEIPKEMTWDLTKIFMDDSEFDLACRQLIEKLKEAETIKNTLKNNATTFLNGIHYLLDIYRKIETIYVYA
HLKNDQDTANTTYQELDAKAGNLYAQVSEAVSWFEPEVLSMPDTQIWQYFKEEPQLEEYRHFIEDILDNRTHVLAADKEA
LLAGASEIFGAPSNIFSILNNADLVFPVIKNEEGKSIQLSHGIYSQLMESVDRNVRKAAYEGFYSVYRQFRNTFASTLGT
HVKTFNYKAKVRNYSSAREAALKSNHIPESVYDTLVKVVDKNLPLLHRYVQLRKKLLKVDKLHMYDMYTPIFCEAPIKFS
YEEAKNEALAALQPLGSEYLDIVKEAFSHRWIDVMENKGKRSGAYSSGSYDTFPYILLNWHDSLDQLYTLVHEMGHSAHS
YFTRKNQPYVYGDYPIFLAEIASTTNENILTEYLLKTETKPQVRAYILNHYLDGFKGTIFRQTQFAEFEQFIHVADANGT
PLTSEYLSENYKKLNRKYYGDAVEPDNEIALEWTRIPHFYYNYYVYQYATGFSAASTLANKIMTQEPHALENYLNYLKSG
SSDYPIEIMKKAGVDMTRPDYIENAMLIFEKRLTELEEITDELIK

Nucleotide


Download         Length: 1818 bp        

>NTDB_id=69844 EP162_RS03250 WP_013773638.1 768832..770649(+) (pepF) [Melissococcus plutonius strain DAT585]
ATGGAGAAAATAAAACAATTACCTAACAGATCTGAGATACCTAAGGAAATGACTTGGGATTTAACAAAAATATTTATGGA
TGATTCGGAATTTGATCTAGCTTGTCGACAGTTAATAGAAAAACTAAAAGAAGCAGAAACAATTAAAAATACGTTAAAAA
ATAATGCAACAACATTTTTAAATGGAATTCATTATCTTTTAGATATTTATCGAAAAATTGAAACTATTTATGTATACGCA
CATTTAAAAAATGATCAAGATACAGCAAATACGACTTATCAAGAATTGGATGCTAAAGCAGGAAATTTATATGCACAAGT
AAGTGAAGCTGTTTCATGGTTTGAACCTGAAGTATTATCAATGCCAGATACACAAATTTGGCAATACTTCAAAGAGGAGC
CTCAATTAGAAGAATATAGACATTTTATTGAAGATATTTTAGACAATCGAACTCATGTCTTAGCTGCTGATAAAGAAGCA
TTACTTGCTGGAGCCAGTGAAATTTTTGGTGCACCTAGCAATATATTTTCTATTTTAAATAATGCAGATTTGGTTTTTCC
AGTTATTAAAAATGAAGAAGGTAAAAGTATTCAATTATCCCATGGCATCTATAGTCAACTAATGGAAAGTGTTGATCGTA
ATGTTAGAAAGGCGGCCTATGAAGGATTTTATAGTGTGTATCGTCAATTTAGAAATACATTTGCATCGACATTAGGAACT
CACGTTAAAACTTTTAATTATAAAGCAAAAGTAAGAAATTATTCATCAGCTAGAGAAGCAGCGTTAAAGAGCAATCATAT
ACCTGAAAGTGTTTATGATACATTGGTAAAAGTAGTTGATAAAAATTTACCATTGCTGCACCGATACGTTCAACTGCGCA
AGAAATTATTAAAAGTAGATAAACTGCATATGTATGATATGTATACGCCTATTTTTTGTGAGGCACCGATTAAATTTTCA
TATGAAGAAGCAAAAAATGAAGCATTAGCTGCATTACAACCGCTTGGCAGTGAGTATCTTGATATAGTAAAAGAAGCATT
TAGTCATCGCTGGATTGATGTAATGGAAAATAAGGGAAAACGAAGTGGTGCTTATTCATCTGGTTCTTATGATACATTTC
CATACATTTTACTTAATTGGCATGATAGTTTAGACCAATTATATACGTTGGTTCATGAGATGGGTCATAGTGCACATAGC
TATTTTACAAGGAAAAATCAACCCTATGTGTATGGTGACTATCCTATCTTTTTAGCAGAGATTGCTTCAACAACCAATGA
AAATATTTTAACAGAATATTTATTAAAGACAGAGACAAAGCCGCAGGTACGCGCCTATATATTGAATCATTATTTAGATG
GCTTTAAAGGGACAATTTTTAGGCAAACTCAATTTGCTGAATTTGAACAGTTTATTCATGTCGCAGATGCAAATGGAACA
CCATTAACAAGTGAATATTTAAGTGAAAATTATAAAAAATTAAATAGAAAATATTATGGAGACGCTGTTGAACCAGACAA
TGAAATTGCATTAGAATGGACACGAATTCCACATTTTTATTACAATTATTATGTTTACCAATACGCAACTGGATTTTCTG
CAGCATCCACTTTAGCAAATAAAATTATGACACAAGAGCCACATGCCTTGGAAAACTATCTGAATTATTTAAAATCAGGT
AGCAGTGATTATCCAATAGAAATAATGAAAAAAGCAGGAGTTGATATGACAAGACCAGATTATATAGAAAATGCTATGTT
AATATTTGAAAAACGTCTTACCGAGTTAGAAGAAATAACAGATGAGTTAATAAAATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(117-186)

Peptidase family M3 (Peptidase_M3)

(207-586)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

53.691

98.512

0.529

Multiple sequence alignment