Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HXS62_RS12330 Genome accession   NZ_AP023230
Coordinates   2603283..2603960 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain YJ4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2598283..2608960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXS62_RS12315 chiA 2599650..2602343 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  HXS62_RS12320 bfd 2602512..2602706 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  HXS62_RS12325 bfr 2602778..2603254 (+) 477 WP_000675504.1 bacterioferritin -
  HXS62_RS12330 pilD 2603283..2603960 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  HXS62_RS12335 gspM 2603960..2604421 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  HXS62_RS12340 gspL 2604418..2605581 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  HXS62_RS12345 gspK 2605596..2606579 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  HXS62_RS12350 gspJ 2606572..2607159 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  HXS62_RS12355 gspI 2607152..2607529 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  HXS62_RS12360 gspH 2607526..2608035 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  HXS62_RS12365 gspG 2608043..2608480 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=69820 HXS62_RS12330 WP_000178161.1 2603283..2603960(-) (pilD) [Escherichia coli strain YJ4]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=69820 HXS62_RS12330 WP_000178161.1 2603283..2603960(-) (pilD) [Escherichia coli strain YJ4]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378