Detailed information NTG0698134

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: NF322_RS00440
• Genome accession: NZ_CP098816
• Coordinates: 100764..102110 (+)
• Length: 448 a.a.
• NCBI ID: WP_000816499.1
• Uniprot ID: Q9R3W9
• Organism: Staphylococcus capitis strain LZD8_nano
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	100764..122252	100764..102110	within	0

Gene organization within MGE regions

Location: 100764..122252

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NF322_RS00440 (NF322_00440)	ccrA	100764..102110 (+)	1347	WP_000816499.1	recombinase family protein	Machinery gene
NF322_RS00445 (NF322_00445)	ccrB	102131..103759 (+)	1629	WP_001186596.1	recombinase family protein	Machinery gene
NF322_RS00450 (NF322_00450)	-	104230..104580 (+)	351	WP_000171449.1	SAUGI family uracil-DNA glycosylase inhibitor	-
NF322_RS00455 (NF322_00455)	-	104665..104976 (+)	312	WP_000833212.1	DUF960 family protein	-
NF322_RS00460 (NF322_00460)	-	104995..105516 (+)	522	WP_000836008.1	DUF1643 domain-containing protein	-
NF322_RS00465 (NF322_00465)	-	105518..105724 (+)	207	WP_001077283.1	hypothetical protein	-
NF322_RS00470 (NF322_00470)	-	105717..106040 (+)	324	WP_030063452.1	JAB domain-containing protein	-
NF322_RS00475 (NF322_00475)	-	106159..107244 (+)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
NF322_RS00480 (NF322_00480)	-	107241..109133 (+)	1893	WP_141519815.1	site-specific integrase	-
NF322_RS00485 (NF322_00485)	-	109140..109517 (+)	378	WP_000361059.1	DUF6262 family protein	-
NF322_RS00490 (NF322_00490)	-	109668..110450 (+)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
NF322_RS00495 (NF322_00495)	erm(A)	110576..111307 (-)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
NF322_RS00500 (NF322_00500)	-	111816..112478 (+)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
NF322_RS00505 (NF322_00505)	-	112823..113461 (-)	639	Protein_98	sulfite exporter TauE/SafE family protein	-
NF322_RS00510 (NF322_00510)	cstR	113461..113721 (-)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
NF322_RS00515 (NF322_00515)	-	113857..114921 (+)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
NF322_RS00520 (NF322_00520)	cstB	114954..116288 (+)	1335	Protein_101	persulfide dioxygenase-sulfurtransferase CstB	-
NF322_RS00525 (NF322_00525)	-	116402..117532 (-)	1131	WP_010922808.1	ROK family transcriptional regulator	-
NF322_RS00530 (NF322_00530)	psm-mec	117865..117933 (+)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
NF322_RS00535 (NF322_00535)	mecI	118018..118389 (-)	372	WP_000369214.1	mecA-type methicillin resistance repressor MecI	-
NF322_RS00540 (NF322_00540)	mecR1	118389..120146 (-)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
NF322_RS00545 (NF322_00545)	mecA	120243..122252 (+)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-

Sequence

Protein

• Length: 448 a.a.
• Molecular weight: 52138.19 Da
• Isoelectric Point: 10.1760

>NTDB_id=698134 NF322_RS00440 WP_000816499.1 100764..102110(+) (ccrA) [Staphylococcus capitis strain LZD8_nano]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide

• Length: 1347 bp

>NTDB_id=698134 NF322_RS00440 WP_000816499.1 100764..102110(+) (ccrA) [Staphylococcus capitis strain LZD8_nano]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (168-250)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-321)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: Q9R3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus COL	78.174	100	0.783
ccrA	Staphylococcus aureus N315	72.383	100	0.725