Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NE424_RS11020 Genome accession   NZ_CP098795
Coordinates   2266111..2266911 (-) Length   266 a.a.
NCBI ID   WP_001984465.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain VB280821     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2261111..2271911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NE424_RS11000 (NE424_11000) - 2262176..2262874 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  NE424_RS11005 (NE424_11005) hisS 2262903..2264195 (-) 1293 WP_031966611.1 histidine--tRNA ligase -
  NE424_RS11010 (NE424_11010) ispG 2264192..2265307 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NE424_RS11015 (NE424_11015) - 2265329..2266120 (-) 792 WP_000405161.1 RodZ domain-containing protein -
  NE424_RS11020 (NE424_11020) pilF 2266111..2266911 (-) 801 WP_001984465.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NE424_RS11025 (NE424_11025) rlmN 2266927..2268159 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  NE424_RS11030 (NE424_11030) ndk 2268282..2268713 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  NE424_RS11035 (NE424_11035) iscX 2268827..2269027 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  NE424_RS11040 (NE424_11040) - 2269127..2269678 (-) 552 WP_004710660.1 phosphatase PAP2 family protein -
  NE424_RS11045 (NE424_11045) - 2269703..2271001 (-) 1299 WP_087937492.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30084.40 Da        Isoelectric Point: 9.2307

>NTDB_id=698061 NE424_RS11020 WP_001984465.1 2266111..2266911(-) (pilF) [Acinetobacter baumannii strain VB280821]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=698061 NE424_RS11020 WP_001984465.1 2266111..2266911(-) (pilF) [Acinetobacter baumannii strain VB280821]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722