Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NE421_RS02540 Genome accession   NZ_CP098791
Coordinates   539438..540238 (+) Length   266 a.a.
NCBI ID   WP_001984465.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 280820     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 534438..545238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NE421_RS02515 (NE421_02515) - 535348..536646 (+) 1299 WP_087937492.1 glycosyltransferase family 1 protein -
  NE421_RS02520 (NE421_02520) - 536671..537222 (+) 552 WP_004710660.1 phosphatase PAP2 family protein -
  NE421_RS02525 (NE421_02525) iscX 537322..537522 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  NE421_RS02530 (NE421_02530) ndk 537636..538067 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  NE421_RS02535 (NE421_02535) rlmN 538190..539422 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  NE421_RS02540 (NE421_02540) pilF 539438..540238 (+) 801 WP_001984465.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NE421_RS02545 (NE421_02545) - 540229..541020 (+) 792 WP_000405161.1 RodZ domain-containing protein -
  NE421_RS02550 (NE421_02550) ispG 541042..542157 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NE421_RS02555 (NE421_02555) hisS 542154..543446 (+) 1293 WP_031966611.1 histidine--tRNA ligase -
  NE421_RS02560 (NE421_02560) - 543475..544173 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30084.40 Da        Isoelectric Point: 9.2307

>NTDB_id=698018 NE421_RS02540 WP_001984465.1 539438..540238(+) (pilF) [Acinetobacter baumannii strain 280820]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=698018 NE421_RS02540 WP_001984465.1 539438..540238(+) (pilF) [Acinetobacter baumannii strain 280820]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722