Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NDO76_RS00180 Genome accession   NZ_CP098743
Coordinates   44314..45684 (+) Length   456 a.a.
NCBI ID   WP_251846721.1    Uniprot ID   -
Organism   Enterococcus faecalis strain M9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 39314..50684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDO76_RS00155 (NDO76_00155) - 39804..40271 (-) 468 WP_002383505.1 VOC family protein -
  NDO76_RS00160 (NDO76_00160) - 40336..41283 (+) 948 WP_016615846.1 YafY family protein -
  NDO76_RS00165 (NDO76_00165) - 41375..42628 (-) 1254 WP_016615845.1 glutamate-5-semialdehyde dehydrogenase -
  NDO76_RS00170 (NDO76_00170) proB 42615..43430 (-) 816 WP_002389550.1 glutamate 5-kinase -
  NDO76_RS00175 (NDO76_00175) - 43750..44235 (+) 486 WP_016615844.1 dUTP diphosphatase -
  NDO76_RS00180 (NDO76_00180) radA 44314..45684 (+) 1371 WP_251846721.1 DNA repair protein RadA Machinery gene
  NDO76_RS00185 (NDO76_00185) - 45788..46933 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  NDO76_RS00190 (NDO76_00190) ispF 46952..47425 (+) 474 WP_016615843.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  NDO76_RS00195 (NDO76_00195) gltX 47478..48935 (+) 1458 WP_010706819.1 glutamate--tRNA ligase -
  NDO76_RS00200 (NDO76_00200) epsC 49205..49744 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49785.33 Da        Isoelectric Point: 7.7031

>NTDB_id=697625 NDO76_RS00180 WP_251846721.1 44314..45684(+) (radA) [Enterococcus faecalis strain M9]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVVPKKEPRVKTELVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMRTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=697625 NDO76_RS00180 WP_251846721.1 44314..45684(+) (radA) [Enterococcus faecalis strain M9]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGCGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTTGTACCTAAAAAAGAGCCGCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCATTAGTTTTAATCGGTGGCGATCCCGGAATTGGTAAGTCAACATTGCT
CTTACAAGTATCACAACAATTAGCAGCCACAGGTGGCACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTACGAGCGGAGCGTTTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATATCACGA
GCCATTGAAAAATTAGAACCAGACTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCCGAATTATTGAAACTGGCAAAAACGAATGGGATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGACCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATCTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCGGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGAGCCACCGGAT
CTTCGATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTATTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCGGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATCCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGGATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

75.165

99.781

0.75
  radA Streptococcus pneumoniae Rx1

75.165

99.781

0.75
  radA Streptococcus pneumoniae D39

75.165

99.781

0.75
  radA Streptococcus pneumoniae R6

75.165

99.781

0.75
  radA Streptococcus pneumoniae TIGR4

75.165

99.781

0.75
  radA Streptococcus mitis SK321

75.165

99.781

0.75
  radA Bacillus subtilis subsp. subtilis str. 168

66.519

98.904

0.658